BBa_M34014BBa_M34014 Version 1 (Component)Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana
BBa_K737049BBa_K737049 Version 1 (Component)The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42
BBa_K737048BBa_K737048 Version 1 (Component)The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42
BBa_K737041BBa_K737041 Version 1 (Component)galK::GFP generator ligated to LacI generator and constitutive sRNA device
BBa_K737040BBa_K737040 Version 1 (Component)galK::GFP generator ligated to TetR generator and constitutive sRNA device
BBa_K1956013BBa_K1956013 Version 1 (Component)gRNA for Kanamycin resistance gene under lambda pL hybrid promoter with terminator
BBa_K1963002BBa_K1963002 Version 1 (Component)A template for expression of sRNA fusions to the E. coli micC hairpin
BBa_K382069BBa_K382069 Version 1 (Component)intron hairpin RNAi knockdown of Bet v 1 allergen in Kan plant vector
BBa_J64852BBa_J64852 Version 1 (Component)DNA stretch between OmpR sigma 70 promoter -10 site and start of transcription
BBa_K382068BBa_K382068 Version 1 (Component)intron hairpin RNAi knockdown of Bet v 1 allergen in Basta plant vector
BBa_K1124104BBa_K1124104 Version 1 (Component)clock device (plux/tet) (-luxI, -tetR, -aiiA, --sRNA, +amilGFP)(AHL sensor)
BBa_K1952012BBa_K1952012 Version 1 (Component)Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter
BBa_K1363604BBa_K1363604 Version 1 (Component)key of no of RNA logic gates
BBa_K737050BBa_K737050 Version 1 (Component)The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42
BBa_K1124102BBa_K1124102 Version 1 (Component)clock device (plux/tet) (+luxI, -tetR, -aiiA, --sRNA, +amilGFP)(positive feedback only)
BBa_K1178000BBa_K1178000 Version 1 (Component)tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon
BBa_K1124103BBa_K1124103 Version 1 (Component)clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only)
BBa_K1179017BBa_K1179017 Version 1 (Component)A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation
BBa_K1179008BBa_K1179008 Version 1 (Component)A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation
BBa_K581003BBa_K581003 Version 1 (Component)SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
iGEM 2019 PlatesiGEM_2019_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2019
iGEM 2018 PlatesiGEM_2018_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2018
BBa_K1124100BBa_K1124100 Version 1 (Component)clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)
BBa_K323151BBa_K323151 Version 1 (Component)DNA program 123456
BBa_K323152BBa_K323152 Version 1 (Component)DNA Program 12346
BBa_K801032BBa_K801032 Version 1 (Component)GAL4 DNA binding domain
BBa_K105101BBa_K105101 Version 1 (Component)Gal4 - DNA binding domain
BBa_K105100BBa_K105100 Version 1 (Component)LacI - DNA binding domain
BBa_K887011BBa_K887011 Version 1 (Component)DNA program : specific DNA sequence which can be recognized by zinc fingers
BBa_K199091BBa_K199091 Version 1 (Component)pLac-CUACC Suppressor tRNA
BBa_K779120BBa_K779120 Version 1 (Component)RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock
BBa_J119088BBa_J119088 Version 1 (Component)pT7-RBS-GFP-tRNA (K191)
BBa_J119087BBa_J119087 Version 1 (Component)pT7-RBS-GFP-tRNA (K191)
CmsRNABBa_K1960102 Version 1 (Component)sRNA, which can silence chloramphenicol resistance
BBa_K831011BBa_K831011 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K831012BBa_K831012 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K199170BBa_K199170 Version 1 (Component)T7 Promoter RBS RFP pLac CCACC (tRNA)
BBa_K199169BBa_K199169 Version 1 (Component)T7 Promoter RBS RFP pLac CUAGC (tRNA)
BBa_K187254BBa_K187254 Version 1 (Component)folD, ORF, forward primer
BBa_K1441013BBa_K1441013 Version 1 (Component)DNA ligase from Escherichia coli with His-tag INSERT
BBa_K809710BBa_K809710 Version 1 (Component)GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator
BBa_K779122BBa_K779122 Version 1 (Component)Short DNA reporter bottom strand (with ROX fluorophore) MammoBlock
BBa_K779121BBa_K779121 Version 1 (Component)Short DNA reporter top strand (with RQ quencher) MammoBlock
PchiP-lacZBBa_K564002 Version 1 (Component)Chitoporin fused with lacZ - target for sRNA based regulation
BBa_K199070BBa_K199070 Version 1 (Component)I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC
BBa_K1942000BBa_K1942000 Version 1 (Component)A shRNA corresponding DNA sequence for KRAS which could silence the gene
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
BBa_K199071BBa_K199071 Version 1 (Component)I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.