BBa_K415007
BBa_K415007 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : pLux/cI-OR : RBS-luxI : Term : p(tetR) : RBS-luxR : Term

BBa_J70404
BBa_J70404 Version 1 (Component)
R0010.E0021.J70394.E0031 or J70398.E0031

BBa_J70403
BBa_J70403 Version 1 (Component)
R0010.E0031.J70394.E0021 or J70397.E0021

BBa_K2123206
BBa_K2123206 Version 1 (Component)
Strong RBS + Organomercurial Lyase (MerB) for E. coli

BBa_M50016
BBa_M50016 Version 1 (Component)
Promoter, RBS and GolS Activator for Gold Detection

BBa_K648131
BBa_K648131 Version 1 (Component)
Pr of Or tester with cI repressor

BBa_K327017
BBa_K327017 Version 1 (Component)
lux OR delta cI activated "off" part

BBa_K812132
BBa_K812132 Version 1 (Component)
mCFP with kozak sequence for expression in Xenopus

BBa_K541000
BBa_K541000 Version 1 (Component)
Limulus anti-LPS factor (LALF) for Bacillus subtilis

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K077025
BBa_K077025 Version 1 (Component)
HSL/LuxR or LasR promotor test system

BBa_J70368
BBa_J70368 Version 1 (Component)
I13502 or K093005 (B0034+E1010) rfp without PsrI sites

Holin, T4
BBa_K112003 Version 1 (Component)
T4 holin, without start or stop codon, berkeley standard

BBa_K415004
BBa_K415004 Version 1 (Component)
P_const(high) : RBS : LuxR : Term : pLux/CI-OR

BBa_J329004
BBa_J329004 Version 1 (Component)
Quorum Sensing Sender Part for Use in BBa_J329003

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_M31748
BBa_M31748 Version 1 (Component)
Gene VII w/o Genes IX RBS or VIII Promoter

BBa_J70552
BBa_J70552 Version 1 (Component)
J70551.J70549 (or J70548.J70550.J70549) composite part

BBa_K750108
BBa_K750108 Version 1 (Component)
PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose

BBa_K2123301
BBa_K2123301 Version 1 (Component)
Novel Synthetic Phytochelatin codon optimized for E. coli expression

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_M31002
BBa_M31002 Version 1 (Component)
Refactored Gene 2 without Gene 10's Promoter or RBS

BBa_K1363611
BBa_K1363611 Version 1 (Component)
key-1 of gate-of-or of RNA logic gates

BBa_K415003
BBa_K415003 Version 1 (Component)
P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR

BBa_K112222
BBa_K112222 Version 1 (Component)
{< ihfB >} The ihf beta gene without start or stop codons, BBb format

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_J70556
BBa_J70556 Version 1 (Component)
BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K812130
BBa_K812130 Version 1 (Component)
Citrine reporter with a Kozak sequence for expression in Xenopus

BBa_K812120
BBa_K812120 Version 1 (Component)
IaaH on PSC2+ Ready for use in xenopus tropicalis egg

BBa_K09100
BBa_K09100 Version 1 (Component)
Receiver for AHL and Outputs GFP when AHL is present

BBa_K1633002
BBa_K1633002 Version 1 (Component)
MOR siRNA 3

BBa_K1633000
BBa_K1633000 Version 1 (Component)
MOR siRNA 1

BBa_K1633001
BBa_K1633001 Version 1 (Component)
MOR siRNA 2

BBa_K648125
BBa_K648125 Version 1 (Component)
Or tester with cI repressor with slightly weak RBS

BBa_K648126
BBa_K648126 Version 1 (Component)
Or tester with cI repressor with very weak RBS

BBa_K251000
BBa_K251000 Version 1 (Component)
coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)

BBa_I714001
BBa_I714001 Version 1 (Component)
trwC of R388 ( relaxase or nickase of the R388 conjugative plasmid )

BBa_K415002
BBa_K415002 Version 1 (Component)
P_const(high) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term

xis domain
BBa_K112203 Version 1 (Component)
{< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21

BBa_J70559
BBa_J70559 Version 1 (Component)
N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040

BBa_K363002
BBa_K363002 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_K1440002
BBa_K1440002 Version 1 (Component)
part1 or our whole design,SV40>PTRE>EYFP>loxP>Ribozyme>SV40

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>