BBa_K782019
BBa_K782019 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_fLuciferase

BBa_K782020
BBa_K782020 Version 1 (Component)
10x[TALA] operator_CMV promoter_fLuciferase

BBa_K782021
BBa_K782021 Version 1 (Component)
10x[TALB] operator_CMV promoter_fLuciferase

BBa_K782022
BBa_K782022 Version 1 (Component)
12x[TALD] operator_CMV promoter_fLuciferase

gpS
BBa_K836004 Version 1 (Component)
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K782023
BBa_K782023 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_fLuciferase

BBa_K782024
BBa_K782024 Version 1 (Component)
2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine

BBa_K782025
BBa_K782025 Version 1 (Component)
4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine

BBa_K782026
BBa_K782026 Version 1 (Component)
7x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782027
BBa_K782027 Version 1 (Component)
7x[TALA] operator_minimal promoter_mCitrine

BBa_K782028
BBa_K782028 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782029
BBa_K782029 Version 1 (Component)
10x[TALA] operator_minimal promoter_mCitrine

BBa_K113007
BBa_K113007 Version 1 (Component)
pBad/araC->T7 polymerase

BBa_K113008
BBa_K113008 Version 1 (Component)
T7 polymerase

BBa_K113010
BBa_K113010 Version 1 (Component)
overlapping T7 promoter

BBa_K113011
BBa_K113011 Version 1 (Component)
more overlapping T7 promoter

BBa_K113012
BBa_K113012 Version 1 (Component)
weaken overlapping T7 promoter

BBa_K113013
BBa_K113013 Version 1 (Component)
pT7->GFP

BBa_K113014
BBa_K113014 Version 1 (Component)
truncated T7 promoter->GFP

BBa_K113015
BBa_K113015 Version 1 (Component)
bidirectional intact T7 promoters->GFP/RFP

BBa_K113016
BBa_K113016 Version 1 (Component)
overlapping T7 promoter->GFP/RFP

BBa_K113017
BBa_K113017 Version 1 (Component)
more overlapping T7 promoter->GFP/RFP

BBa_K113018
BBa_K113018 Version 1 (Component)
weaken overlapping T7 promoter->GFP/RFP

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

T7Gene6
BBa_K093001 Version 1 (Component)
T7 Gene 6 exonuclease

BBa_K098986
BBa_K098986 Version 1 (Component)
lac QPI driven by high promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098981
BBa_K098981 Version 1 (Component)
tet QPI driven by high promoter

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

mtGFP
BBa_K809001 Version 1 (Component)
Mitochondrial GFP

BBa_K782030
BBa_K782030 Version 1 (Component)
10x[TALB] operator_minimal promoter_mCitrine

BBa_K782031
BBa_K782031 Version 1 (Component)
12x[TALD] operator_minimal promoter_mCitrine

nitR
BBa_K836007 Version 1 (Component)
Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

BBa_K1982002
BBa_K1982002 Version 1 (Component)
Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K113004
BBa_K113004 Version 1 (Component)
pT7->tetR operator->GFP

BBa_S04136
BBa_S04136 Version 1 (Component)
terminator and cI promoter

BBa_K098996
BBa_K098996 Version 1 (Component)
mtrB with terminator

BBa_K113005
BBa_K113005 Version 1 (Component)
pLac->tetR->pT7->tetR operator ->GFP

BBa_K098994
BBa_K098994 Version 1 (Component)
mtrB with RBS and terminator

BBa_K105020
BBa_K105020 Version 1 (Component)
tetR - operator

BBa_K105021
BBa_K105021 Version 1 (Component)
cI - operator

BBa_K105022
BBa_K105022 Version 1 (Component)
lex A - operator

BBa_K105023
BBa_K105023 Version 1 (Component)
lac I - operator

BBa_K105024
BBa_K105024 Version 1 (Component)
Gal4 - operator

BBa_K105025
BBa_K105025 Version 1 (Component)
Adh1 terminator