SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

BBa_J61048
BBa_J61048 Version 1 (Component)
[rnpB-T1] Terminator

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

mRFPr
BBa_J31008 Version 1 (Component)
RFP reverse

BBa_J61002
BBa_J61002 Version 1 (Component)
EX-Ptet-S-rbsRFP-P "RFP reporter"

BBa_K1017811
BBa_K1017811 Version 1 (Component)
Pcons+rRBS-2+mRFP+J61048

Cre
BBa_J61047 Version 1 (Component)
Cre DNA recombinase

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

BBa_J52008
BBa_J52008 Version 1 (Component)
luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)

BBa_K199118
BBa_K199118 Version 1 (Component)
T7 promoter + RBS + RFP

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

mRFP
BBa_K1615089 Version 1 (Component)
mRFP RFC25

mRFP1
BBa_E2050 Version 1 (Component)
derivative of mRFP1, yeast-optimized

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

CmR
BBa_J61000 Version 1 (Component)
chloramphenicol resistance cassette

BBa_I13521
BBa_I13521 Version 1 (Component)
Ptet mRFP

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

Key 1
BBa_J01008 Version 1 (Component)
Riboregulator key 1

pCon 0.36
BBa_K176009 Version 1 (Component)
constitutive promoter family member J23107 actual sequence (pCon 0.36)

BBa_K1114107
BBa_K1114107 Version 1 (Component)
Bicistronic Design RBS 2, MoClo Format with BC fusion sites

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

pCon 0.15
BBa_K176008 Version 1 (Component)
constitutive promoter family member J23115 actual sequence (pCon 0.15)

mRFP1
BBa_J04650 Version 1 (Component)
E1010.B0015

BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)

mRFP1
BBa_I13502 Version 1 (Component)
Screening plasmid intermediate

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_I715039
BBa_I715039 Version 1 (Component)
pLac-RBS-RFP

BBa_K1491009
BBa_K1491009 Version 1 (Component)
RBS-mRFP-His

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_I714075
BBa_I714075 Version 1 (Component)
[J01008][B0015] ([Key1][Double Terminator])

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

RFP2
BBa_I715023 Version 1 (Component)
Carboxyl portion of RFP