BBa_K323005BBa_K323005 Version 1 (Component)CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system
BBa_K323021BBa_K323021 Version 1 (Component)CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system
BBa_K1088016BBa_K1088016 Version 1 (Component)HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)
BBa_K1123006BBa_K1123006 Version 1 (Component)P(RG) Protein Production Construct
BBa_M10034BBa_M10034 Version 1 (Component){˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter
BBa_K874103BBa_K874103 Version 1 (Component)Arabinose inducible expression of PZF3838, M.ScaI Fusion protein (ARA -> PZF3838, M.ScaI FP)
BBa_K359005BBa_K359005 Version 1 (Component)Senses AIP and has RFP reporter (from Agr quorum sensing system)
ArsR Gen.BBa_K935001 Version 1 (Component)E. coli chromosomal ars promoter with arsR repressor gene and double terminator (B0010-B0012)
BBa_K323080BBa_K323080 Version 1 (Component)CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system
BBa_K323029BBa_K323029 Version 1 (Component)CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system
BBa_M45091BBa_M45091 Version 1 (Component)AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa
BBa_J70593BBa_J70593 Version 1 (Component)RFC12 ATG Head Domain
BBa_M31539BBa_M31539 Version 1 (Component)These are the genes in M13K07 responsible for forming the 'tip' of the phage.
BM-RFPBBa_K774005 Version 1 (Component)Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B
BBa_K1051801BBa_K1051801 Version 1 (Component)Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib
BBa_K1362300BBa_K1362300 Version 1 (Component)NpuDnaE N-Intein RFC[105] assembly construct (with His6)
BBa_M36120BBa_M36120 Version 1 (Component)5' Bicistronic UTR (strong) contains ATG start Codon.
no ATG GFPBBa_K579000 Version 1 (Component)no ATG GFP
Plac/ara-1BBa_K1145006 Version 1 (Component)Drive the expression of LuxRI in pLuxRI2 and its derivatives.
BBa_J70498BBa_J70498 Version 1 (Component)Red {-1,4;0,5} PsrI part (for non protein/AG parts)
BBa_K1961000BBa_K1961000 Version 1 (Component)CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli
BBa_M10035BBa_M10035 Version 1 (Component){˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain
BBa_K329043BBa_K329043 Version 1 (Component)Right arm Tn916 - Mutant + Mutant Lambda
BBa_J58009BBa_J58009 Version 1 (Component)Fusion protein Trg-EnvZ
BBa_I716015BBa_I716015 Version 1 (Component)RFP without start ATG
BBa_K323075BBa_K323075 Version 1 (Component)ATG cYFP link HIVC
BBa_K329042BBa_K329042 Version 1 (Component)Right arm Tn916 - Wild Type + Mutant Lambda
BBa_I720010BBa_I720010 Version 1 (Component)Ara landing pad (pBBLP 8)
BBa_K299800BBa_K299800 Version 1 (Component)standard biobrick scar (if next part starts with ATG)
BBa_K1447004BBa_K1447004 Version 1 (Component)Epitope 1-5 from Ara h 1
BBa_K1447005BBa_K1447005 Version 1 (Component)Epitope 1-5 from Ara h 1
BBa_M36556BBa_M36556 Version 1 (Component)5' Bicistronic UTR (medium), does not include ATG start
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.