PetroBrickBBa_K590025 Version 1 (Component)The PetroBrick: Strong Constitutive Expression of ADC and AAR in pSB1C3
BBa_K812021BBa_K812021 Version 1 (Component)Eukaryotic PoPs -> IaaM. Indoleacetamide monooxygenase for Auxin generator, with kozak
BBa_K1022129BBa_K1022129 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022128BBa_K1022128 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022127BBa_K1022127 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys
BBa_K176238BBa_K176238 Version 1 (Component)High input score automatic selector: PoPS->luxI-LVA->K176227
BBa_K176244BBa_K176244 Version 1 (Component)For characterization of K176240: VR9+pCon 0.15->K176240->PoPS
BBa_K176239BBa_K176239 Version 1 (Component)High input score automatic selector: PoPS->luxI-LVA->K176228
BBa_K176243BBa_K176243 Version 1 (Component)For characterization of K176240: VR7+pCon 0.36->K176240->PoPS
BBa_K176245BBa_K176245 Version 1 (Component)For characterization of K176240: VR5+pCon 0.01->K176240->PoPS
BBa_K143073BBa_K143073 Version 1 (Component)AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)
BBa_I13266BBa_I13266 Version 1 (Component)Lux-based Sender/Receiver Device which outputs PoPS
BBa_K748005BBa_K748005 Version 1 (Component)P2+RBS+GFP+T. P2 can be activated by phosphorylated AgrA.
BBa_K748004BBa_K748004 Version 1 (Component)P3+RBS+GFP+T. P3 can be activated by phosphorylated AgrA.
BBa_K176229BBa_K176229 Version 1 (Component)High input score automatic selector: PoPS->luxI-LVA->K176226
BBa_J37037BBa_J37037 Version 1 (Component)This device is to test the Pops Blocker part
BBa_K176241BBa_K176241 Version 1 (Component)High input score automatic selector (with reporter): PoPS->GFP->K176238
BBa_K176242BBa_K176242 Version 1 (Component)High input score automatic selector (with reporter): PoPS->GFP->K176239
BBa_K176246BBa_K176246 Version 1 (Component)High input score automatic selector (with reporter): PoPS->mRFP->K176229
BBa_J37038BBa_J37038 Version 1 (Component)This device is to test the Pops Blocker part
BBa_J13001BBa_J13001 Version 1 (Component)LuxR generator. POPS generate cI(LVA) and LuxI(LVA)
BBa_K359002BBa_K359002 Version 1 (Component)Agr quroum sensing sensor/generator, FepA pore, with P2 + reporter
BBa_K748001BBa_K748001 Version 1 (Component)AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
BBa_I722012BBa_I722012 Version 1 (Component)AHL->RoPs and mRFP producer
BBa_K1351036BBa_K1351036 Version 1 (Component)Quorum sensing two component system (AIP-II sensing histidine kinase agrC, response regulator agrA)
ADHAcBBa_M36203 Version 1 (Component)ADH Actuator; input is PoPS and output is ADH enzyme
BBa_M36222BBa_M36222 Version 1 (Component)ArsAction: Arsenite Removal Device (input: PoPs Signal, output: removal device)
BBa_M45091BBa_M45091 Version 1 (Component)AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa
BBa_K1452002BBa_K1452002 Version 1 (Component)Pos/neg selection (Bacillus)
BBa_K1452001BBa_K1452001 Version 1 (Component)Pos/neg selection (Bacillus)
BBa_K359005BBa_K359005 Version 1 (Component)Senses AIP and has RFP reporter (from Agr quorum sensing system)
BBa_K176172BBa_K176172 Version 1 (Component)Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFP
BBa_K176115BBa_K176115 Version 1 (Component)Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)
BBa_K176248BBa_K176248 Version 1 (Component)Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229
BBa_K176091BBa_K176091 Version 1 (Component)Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)
BBa_K176249BBa_K176249 Version 1 (Component)Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240
BBa_K1621009BBa_K1621009 Version 1 (Component)pOP - protein expression vector compatible with RFC[25]
BBa_K106697BBa_K106697 Version 1 (Component)AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
BBa_K106679BBa_K106679 Version 1 (Component)GFP, under pheromone-inducible promoter, 5' LexA Ops
BBa_M36459BBa_M36459 Version 1 (Component)PoPS -> Cell Lysis
BBa_M36458BBa_M36458 Version 1 (Component)PoPS -> Cell Lysis
BBa_M34567BBa_M34567 Version 1 (Component)converts PoPS to foobob
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_K1452000BBa_K1452000 Version 1 (Component)Pos/neg selection (Bacillus)
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.