BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K300097
BBa_K300097 Version 1 (Component)
Triple phasin separated by a flexible protein domain linker

BBa_K1711000
BBa_K1711000 Version 1 (Component)
AFB2 auxin-binding F-box protein

ZF7
BBa_K655006 Version 1 (Component)
Zinc Finger Protein (ZF7) (binding site:GACGGCGCC)

BBa_K1094000
BBa_K1094000 Version 1 (Component)
Enhanced flavin-binding fluorescent protein (eFbFP)

ZF6
BBa_K655005 Version 1 (Component)
Zinc Finger Protein (ZF6) (binding site:GACGTAGGA)

ZF8
BBa_K655007 Version 1 (Component)
Zinc Finger Protein (ZF8) (binding site:TGTGTGGAG)

BBa_K411105
BBa_K411105 Version 1 (Component)
eIF4A binding aptamer

TAL
BBa_K1129000 Version 1 (Component)
Tyrosine Ammonia Lyase (TAL) from Rhodobacter sphaeroides

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1314016
BBa_K1314016 Version 1 (Component)
SH3 Domain-Linker-PDZ Domain

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

CDA
BBa_K1266000 Version 1 (Component)
Chitin Deacetylase or CDA2

BBa_K931002
BBa_K931002 Version 1 (Component)
A Red Fluorescent Protein - Starch Binding Protein Fusion

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K1976048
BBa_K1976048 Version 1 (Component)
DNase domain of Colicin E2

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K931009
BBa_K931009 Version 1 (Component)
An L-Arabinose Controlled Thiamine Binding - Starch Binding Protein Fusion

AG4
BBa_M10023 Version 1 (Component)
{˂AG4˃} Silver binding peptide

BBa_M11076
BBa_M11076 Version 1 (Component)
NrtA: nitrate/nitrite binding protein coding region

BBa_M11080
BBa_M11080 Version 1 (Component)
silE (coding sequence for Silver binding protein)

BBa_M11081
BBa_M11081 Version 1 (Component)
cutA (coding sequence for copper binding protein)

BBa_K150005
BBa_K150005 Version 1 (Component)
ribosome binding site of pTrc99a

BBa_J56006
BBa_J56006 Version 1 (Component)
Codon-optimized signaling domain, cytoplasmic domain of Tar

BBa_K371056
BBa_K371056 Version 1 (Component)
ATG

GFP-AID
BBa_K812010 Version 1 (Component)
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)

BBa_K1909004
BBa_K1909004 Version 1 (Component)
NOD1 LRR Domain

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K165044
BBa_K165044 Version 1 (Component)
Zif268-HIV DNA binding domain with SV40 NLS and ADH1 terminator

BBa_K1675001
BBa_K1675001 Version 1 (Component)
NhaB; sodium-proton antiporter; membrane protein

BBa_K1675000
BBa_K1675000 Version 1 (Component)
NhaA; sodion-proton antiporter; membrane protein