BBa_K102908BBa_K102908 Version 1 (Component)TA10 gate from synthetic algorithm v1.1
BBa_K102909BBa_K102909 Version 1 (Component)TA11 gate from synthetic algorithm v1.1
BBa_K102910BBa_K102910 Version 1 (Component)TA12 gate from synthetic algorithm v1.1
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
LuxO D47EBBa_K131022 Version 1 (Component)LuxO D47E, Vibrio harveyi
LuxO D47ABBa_K131023 Version 1 (Component)LuxO D47A, Vibrio harveyi
BBa_K102911BBa_K102911 Version 1 (Component)TA13 gate from synthetic algorithm v1.2
p_qrr4BBa_K131017 Version 1 (Component)p_qrr4 from <I>Vibrio harveyi</I>
rTT+pRBBa_K093003 Version 1 (Component)Convergent Promoter System: Forward Module: rTT+lambda pR
BBa_K102905BBa_K102905 Version 1 (Component)TA7 gate from synthetic algorithm v1.1
rPlac+TTBBa_K093004 Version 1 (Component)Convergent Promoter System: Reverse Module: rPlac+TT
BBa_S04147BBa_S04147 Version 1 (Component)The Partial Butanol Operon (5 inserts from I725021 to I725025)
BBa_K082017BBa_K082017 Version 1 (Component)general recombine system
RBS.TetRBBa_I15040 Version 1 (Component)RBS.TetR from pASKIBA3
BBa_K116000BBa_K116000 Version 1 (Component)A homolog of OCT3 (organic cation transporter 3) from E coli. K12
BBa_K116601BBa_K116601 Version 1 (Component)HtlB (ftsH) coding region from E. coli
BBa_K116401BBa_K116401 Version 1 (Component)external phosphate sensing promoter
BBa_K153007BBa_K153007 Version 1 (Component)Ori 101 from pKD46
BBa_K127003BBa_K127003 Version 1 (Component)Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator
BBa_K089004BBa_K089004 Version 1 (Component)phaC Promoter (-663 from ATG)
BBa_K127013BBa_K127013 Version 1 (Component)gas vesicle protein A from cyanobacteria Anabaena
agrDBBa_K090601 Version 1 (Component)AgrD: AGR System AIP Precursor
BBa_K116404BBa_K116404 Version 1 (Component)external phosphate sensing reporter
BBa_K116402BBa_K116402 Version 1 (Component)polyphosphate kinase
SV40 NLSBBa_J63008 Version 1 (Component)SV40 nuclear localization sequence from SV40; yeast codon optimized
CIIBBa_K116602 Version 1 (Component)CII coding region from λ phage
pREBBa_K116603 Version 1 (Component)pRE promoter from λ phage
CIIICdBBa_K116609 Version 1 (Component)CIIICd: modified CIII coding region from λ phage
BBa_K152006BBa_K152006 Version 1 (Component)frdBCD operon from E. coli (missing the first gene fumarate oxidase)
BBa_K116201BBa_K116201 Version 1 (Component)ureD promoter from <I>P mirabilis<I>
BBa_K152008BBa_K152008 Version 1 (Component)250 bp fragment from the Zea mays gene TB1
BBa_K112149BBa_K112149 Version 1 (Component)PmgtCB Magnesium promoter from <I>Salmonella</I>
BBa_K177007BBa_K177007 Version 1 (Component)PhoP/PhoQ locus from salmonella typhimurium
BBa_M11005BBa_M11005 Version 1 (Component)comE3 Competence locus E from H. pylori
BBa_M12065BBa_M12065 Version 1 (Component)CBHI from T. Reesei
BBa_M10034BBa_M10034 Version 1 (Component){˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter
BBa_M10035BBa_M10035 Version 1 (Component){˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain
BBa_K216000BBa_K216000 Version 1 (Component)luxB coding sequence from Photobacterium phosphoreum ATCC11060
BBa_K294000BBa_K294000 Version 1 (Component)This is the coding sequence for the heat shock protein hsp15 from E. coli
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
BBa_B0013BBa_B0013 Version 1 (Component)TE from coliphage T7 (+/-)
BBa_K177010BBa_K177010 Version 1 (Component)invasine locus from yersinia
BBa_K177023BBa_K177023 Version 1 (Component)listeriolysin O - locus from Listeria monocytogenes
BBa_I746365BBa_I746365 Version 1 (Component)PLL promoter from P4 phage
BBa_K177026BBa_K177026 Version 1 (Component)listeriolysin O 399G->C - locus from Listeria monocytogenes