Cas9Cas9 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Amp CasBBa_I13800 Version 1 (Component)Ampicillin Resistance Cassette
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
Scar 25BBa_B0105 Version 1 (Component)RFC 25 Scar Sequence
LacIBBa_R0010 Version 1 (Component)promoter (lacI regulated)
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
BBa_J56012BBa_J56012 Version 1 (Component)Invertible sequence of dna includes Ptrc promoter
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_K2082251BBa_K2082251 Version 1 (Component)Sequence for rpoZ Knock-Out in E. coli through CRISPR/Cas9
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
mCherryBBa_J06504 Version 1 (Component)monomeric RFP optimized for bacteria
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
PelBBBa_J32015 Version 1 (Component)PelB leader sequence; directs protein to E. coli periplasmic membrane
scarBBa_K112999 Version 1 (Component)BBb1 scar sequence
FurBO_11498 Version 1 (Component) BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli
RFC 37BBa_K245009 Version 1 (Component)RFC 37 Scar Sequence
Cse1BBa_K1218003 Version 1 (Component)CRISPR CasA E. coli (Modern)
BBa_K2066019BBa_K2066019 Version 1 (Component)UNS 3 Sequence, from Torella et al., 2013
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
BBa_I746916BBa_I746916 Version 1 (Component)superfolder GFP coding sequence
BBa_I746200BBa_I746200 Version 1 (Component)FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
BBa_R0085BBa_R0085 Version 1 (Component)T7 Consensus Promoter Sequence
BBa_K165002BBa_K165002 Version 1 (Component)Kozak sequence (yeast RBS)
HisTagBBa_J18909 Version 1 (Component)His tag (6xHis; E. coli - optimized)