GFPGFP Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
BBa_B0012BBa_B0012 Version 1 (Component)TE from coliphageT7
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
BBa_J04450BBa_J04450 Version 1 (Component)RFP Coding Device
Scar 25BBa_B0105 Version 1 (Component)RFC 25 Scar Sequence
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
GFP reportBBa_E0240 Version 1 (Component)GFP generator
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_K1159303BBa_K1159303 Version 1 (Component)Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
GFP generaBBa_E0840 Version 1 (Component)GFP generator
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
mCherryBBa_J06504 Version 1 (Component)monomeric RFP optimized for bacteria
BBa_K1159315BBa_K1159315 Version 1 (Component)TMD of SERK Receptor from <i>Physcomitrella patens</i> with C-terminal GFP fusion in RFC[25]
BBa_K1159316BBa_K1159316 Version 1 (Component)SERK-Receptor TMD with C-terminal GFP and N-terminal <i>Strep</i>-tag II TEV-site linker in RFC[25]
BBa_K1159305BBa_K1159305 Version 1 (Component)Transmembrane domain of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
RBS-RFPBBa_K093005 Version 1 (Component)RFP with RBS
BBa_K1051003BBa_K1051003 Version 1 (Component)Stop codon free GFP in RFC[23] standard
BBa_K1051000BBa_K1051000 Version 1 (Component)Stop codon free RFP in RFC[23] standard
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
RFC 37BBa_K245009 Version 1 (Component)RFC 37 Scar Sequence
BBa_K249006BBa_K249006 Version 1 (Component)Fusion EYFP with C-terminal Arginine Tag
BBa_K1321005BBa_K1321005 Version 1 (Component)Synthetic Phytochelatin EC(20) RFC[25]