RBS-ho1
BBa_K193400 Version 1 (Component)
RBS is located upstream of ho1

BBa_K1228004
BBa_K1228004 Version 1 (Component)
A fragment of loctoferrin

BBa_K643004
BBa_K643004 Version 1 (Component)
Improved version of Bba_K231000

BBa_K1114203
BBa_K1114203 Version 1 (Component)
C0071_CD Moclo of BBA_C0071

BBa_J100105
BBa_J100105 Version 1 (Component)
Low production of eCDM8

praiI
BBa_K594009 Version 1 (Component)
the promoter of raiI

BBa_M33330
BBa_M33330 Version 1 (Component)
RC of atzR CDS

prhiABC
BBa_K594006 Version 1 (Component)
the promoter of rhiABC

BBa_K321004
BBa_K321004 Version 1 (Component)
intracellular chain of KIR3DL1

BBa_K341709
BBa_K341709 Version 1 (Component)
plasmid of Donor Plasmid

psinI
BBa_K594004 Version 1 (Component)
the promoter of sinI

BBa_K115058
BBa_K115058 Version 1 (Component)
truncated version of BBa_K115052

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_K078003
BBa_K078003 Version 1 (Component)
one part of the initial dioxygenase of the dioxin degradation pathway

pINDEL
BBa_K551001 Version 1 (Component)
plasmid of insertion and deletion

BBa_M31373
BBa_M31373 Version 1 (Component)
First Half of M13K07 g3

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1972008
BBa_K1972008 Version 1 (Component)
dszB with tac promoter induced by IPTG

BBa_K189030
BBa_K189030 Version 1 (Component)
Nin 146 of lambda genome

BBa_K189040
BBa_K189040 Version 1 (Component)
Orf-401 of lambda genome

BBa_K189039
BBa_K189039 Version 1 (Component)
Orf-194 of lambda genome

BBa_K1689003
BBa_K1689003 Version 1 (Component)
Coding sequence of Nluc416-FRB

BBa_K189038
BBa_K189038 Version 1 (Component)
Orf-314 of lambda genome

BBa_K189026
BBa_K189026 Version 1 (Component)
Nin 204 of lambda genome

BBa_K189032
BBa_K189032 Version 1 (Component)
Nin 221 of lambda genome

BBa_K189042
BBa_K189042 Version 1 (Component)
Integration protein of lambda genome

BBa_J75004
BBa_J75004 Version 1 (Component)
RBS-TetR-Ter-lambdaPr-RBS

BBa_K736003
BBa_K736003 Version 1 (Component)
pLAC-regulated TAT-RFP construct

BBa_K1453101
BBa_K1453101 Version 1 (Component)
[ssDsbA]->[RFP]->[Lgt]->[TAL-adapter]

BBa_K1085037
BBa_K1085037 Version 1 (Component)
FliZ Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2

BBa_I712049
BBa_I712049 Version 1 (Component)
CMV + Myc + CCR5 + TIR(TLR3)

BBa_K1085038
BBa_K1085038 Version 1 (Component)
MotB Strep-tag SilkSubunitE1 SilkSubunitE2

BBa_K563057
BBa_K563057 Version 1 (Component)
yeast vector with CIT2-Tor

BBa_K1945005
BBa_K1945005 Version 1 (Component)
C-terminal fragment of T7 RNA Polymerase

BBa_K180002
BBa_K180002 Version 1 (Component)
tac pR testing plasmid (GFP)

BBa_K1647017
BBa_K1647017 Version 1 (Component)
Bac8c,a kind of antimicrobial peptides

BBa_K1702000
BBa_K1702000 Version 1 (Component)
Overexpression of E. coli fadD under control of lac promoter

BBa_K1702001
BBa_K1702001 Version 1 (Component)
Overexpression of E. coli fadL under control of lac promoter

BBa_K1647020
BBa_K1647020 Version 1 (Component)
AAP2, a kind of antimicrobial peptides

BBa_K1781004
BBa_K1781004 Version 1 (Component)
T18 - one of two fragments of the T25/T18 BACTH system

BBa_K1465227
BBa_K1465227 Version 1 (Component)
sRNA of phosphofructokinase A under the control of T7 promoter (T7_sRNA:pfkA)

BBa_K1465226
BBa_K1465226 Version 1 (Component)
sRNA of phosphofructokinase A under the control of ptac promoter (ptac_sRNA:pfkA)

BBa_K1781003
BBa_K1781003 Version 1 (Component)
T25 - one of two fragments of the T25/T18 BACTH system

BBa_K1202107
BBa_K1202107 Version 1 (Component)
TAT-E4-orf4: Cancer killing peptide

BBa_K1989006
BBa_K1989006 Version 1 (Component)
hexaspytag with SUP and His-tag

BBa_K2148010
BBa_K2148010 Version 1 (Component)
mRasberry C.reinhardtii-CO far-red FP

BBa_K1352006
BBa_K1352006 Version 1 (Component)
Ice Nucleation Protein (INP) FLAG-tag

BBa_K1968024
BBa_K1968024 Version 1 (Component)
LacI without LVA-tag (Repressor) Phytobrick

BBa_K2082119
BBa_K2082119 Version 1 (Component)
AraC-Pbad(mod)-RBS-WTPolI-Ter