BBa_K176086
BBa_K176086 Version 1 (Component)
cell density->PoPS: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS

BBa_K176083
BBa_K176083 Version 1 (Component)
cell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS

BBa_I711023
BBa_I711023 Version 1 (Component)
beta-hydroxybutyryl CoA -> Crotonyl ??? CoA (Crotonase)

BBa_K1926011
BBa_K1926011 Version 1 (Component)
The SNAP UNIT: SNAP-tag flanked by loxP

BBa_C100004
BBa_C100004 Version 1 (Component)
ORF of JMT odorant enzyme (Synthesized)

CelC
BBa_K1044000 Version 1 (Component)
Endoglucanase gene CelC from Agrobacterium tumefaciens C58.

BBa_K341002
BBa_K341002 Version 1 (Component)
promoter+tet-SDS

BBa_K079038
BBa_K079038 Version 1 (Component)
TetO-wt/4C5G - operator library member

BBa_K658002
BBa_K658002 Version 1 (Component)
a bacteria population-control device with RBS0.6 driven by lacl+pL

iccdB0.07
BBa_K658005 Version 1 (Component)
a bacteria population-control device with RBS0.07 driven by lacl+pL

BBa_K658004
BBa_K658004 Version 1 (Component)
a bacteria population-control device with RBS0.3 driven by lacl+pL

BBa_K817031
BBa_K817031 Version 1 (Component)
PfadBA-TetR(P0340)-pLac

BBa_K319037
BBa_K319037 Version 1 (Component)
TetR+yeGFP+ADH1 terminator

BBa_K1311006
BBa_K1311006 Version 1 (Component)
K081005+TetR+B0015+pTet

BBa_K510039
BBa_K510039 Version 1 (Component)
pUC18Sfi-miniTn7BB-Gm-tetR

BBa_K1819004
BBa_K1819004 Version 1 (Component)
GFP+TetR+pTetR+mRFP1

BBa_K209418
BBa_K209418 Version 1 (Component)
SSFYFP-hM4D-Beta Pix

BBa_K371029
BBa_K371029 Version 1 (Component)
MPF(meta-prefix)+pduV98

BBa_K1132022
BBa_K1132022 Version 1 (Component)
BBa_J23116-TetR-pTet-RFP

BBa_K209422
BBa_K209422 Version 1 (Component)
SSFYFP-Rs1.3-Beta Pix

BBa_K209431
BBa_K209431 Version 1 (Component)
SSFYFP-hM2D-Beta Pix

BBa_K1124220
BBa_K1124220 Version 1 (Component)
mCherry-tetR-aiiA (+d.term)

BBa_K133050
BBa_K133050 Version 1 (Component)
TetR-CF-RGD-Histop

BBa_K817032
BBa_K817032 Version 1 (Component)
PfadBA-TetR(P1040)-pLac

BBa_K1387004
BBa_K1387004 Version 1 (Component)
pIBPAB - B0034 - TetR - B0015

BBa_K336008
BBa_K336008 Version 1 (Component)
p(tetR)+RBS+lasl

BBa_K1741002
BBa_K1741002 Version 1 (Component)
sfGFP under a short arabinose promoter s2 without O1 and O2

BBa_K1189001
BBa_K1189001 Version 1 (Component)
TALE-B with a his 6 tag under a lacI promoter

pBAD-AB-DT
BBa_K323135 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter

BBa_K112400
BBa_K112400 Version 1 (Component)
Promoter for grpE gene - Heat Shock and Ultrasound Sensitive

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1316009
BBa_K1316009 Version 1 (Component)
yqjF promoter coupled to mKate2 reporter gene and ybiJ promoter coupled to mKate2 reporter gene

BBa_K1480004
BBa_K1480004 Version 1 (Component)
TetR generator (Promoter J23106 )

BBa_K1480005
BBa_K1480005 Version 1 (Component)
TetR generator (Promoter J23111 )

BBa_K1480003
BBa_K1480003 Version 1 (Component)
TetR generator (Promoter J23109)

BBa_K283049
BBa_K283049 Version 1 (Component)
pLacI-rbs-tetR(LVA)

BBa_K336018
BBa_K336018 Version 1 (Component)
p(tetR),LuxI and LuxR

BBa_K173005
BBa_K173005 Version 1 (Component)
tetR logic inverter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K2110007
BBa_K2110007 Version 1 (Component)
TPA transporting protein gene expression system

BisdAB
BBa_K525517 Version 1 (Component)
Fusion protein of BisdA and BisdB behind constitutive promoter

BBa_J24804
BBa_J24804 Version 1 (Component)
This is Renilla Luciferase under constitutive promoter with two terminators.

BBa_K563003
BBa_K563003 Version 1 (Component)
promoter of CIT2

BBa_I12222
BBa_I12222 Version 1 (Component)
Wild-type TetR(B) protein generator

BBa_J23039
BBa_J23039 Version 1 (Component)
[TetR]+[rbs][LuxI][dblTerm] "Constitutive luxI"

BBa_K1631002
BBa_K1631002 Version 1 (Component)
Translational unit of Colicin-E3