CBDcipA
BBa_K1615111 Version 1 (Component)
CBDcipA with N and C-terminal linker in RFC25

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

s1+s2+s3
BBa_K2027045 Version 1 (Component)
Long Bacterial Collagen Fiber Monomer without Cross-linking Domains

BBa_K1321014
BBa_K1321014 Version 1 (Component)
CBDCipA with N and C-terminal linker

BBa_K299107
BBa_K299107 Version 1 (Component)
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)

Zif23
BBa_R2002 Version 1 (Component)
Promoter, Zif23 regulated, test: between and after

BBa_M1014
BBa_M1014 Version 1 (Component)
Complete construct for phasin secretion together with zinc finger binding sites

BBa_I13545
BBa_I13545 Version 1 (Component)
GFP and RFP polycistronic

BBa_K2144009
BBa_K2144009 Version 1 (Component)
Sequence for His6-tag and LPXTGG-tag

BBa_K861022
BBa_K861022 Version 1 (Component)
FadE with RBS and terminator

BBa_K887051
BBa_K887051 Version 1 (Component)
Ptet(promoter)+B0032(RBS)+Gli1(zinc finger)+kivD(enzyme)+B0015(terminator)

BBa_K1833011
BBa_K1833011 Version 1 (Component)
lac promoter -> Zinc finger 225 binding domain fused to immunogenic MUC1 peptide

BBa_K574005
BBa_K574005 Version 1 (Component)
3OC12HSL and YFP regulated by pBad

BBa_K081024
BBa_K081024 Version 1 (Component)
pcyA protein generator and Pomp

BBa_K390001
BBa_K390001 Version 1 (Component)
5'UTR and native RBS from psbA2 in Synechocystis sp. PCC 6803

BBa_K390002
BBa_K390002 Version 1 (Component)
5'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 6803

BBa_K649000
BBa_K649000 Version 1 (Component)
LasR and 3OC12HSL regulated promoter.

BBa_K1123012
BBa_K1123012 Version 1 (Component)
His-Tag and Thrombine Cleavage Site

BBa_K1758312
BBa_K1758312 Version 1 (Component)
Chromium responsive promoter with UTR+RBS and sfGFP

BBa_K2130004
BBa_K2130004 Version 1 (Component)
Report plasmid with toxin ccdB gene and inducible BAD promoter

rhII
BBa_C0170 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL (no LVA)

NAND2616
BBa_I732301 Version 1 (Component)
NAND Candidate (U073O26D001O16)

BBa_I741018
BBa_I741018 Version 1 (Component)
Right facing promoter (for xylF) controlled by xylR and CRP-cAMP

BBa_K1361000
BBa_K1361000 Version 1 (Component)
Curli Fiber generator under Pbad induction with positive regulation

NAND2717
BBa_I732302 Version 1 (Component)
NAND Candidate (U073O27D001O17)

BBa_M36084
BBa_M36084 Version 1 (Component)
Histidine Tag and Stop Codon

BBa_K2116004
BBa_K2116004 Version 1 (Component)
AND gate regulated by norR and esaR (one esabox)

BBa_I746315
BBa_I746315 Version 1 (Component)
arabinose induced expression of phiR73 delta and mRFP

BBa_I746313
BBa_I746313 Version 1 (Component)
arabinose induced expression of P2 ogr and mRFP

NAND2-7
BBa_I732304 Version 1 (Component)
NAND Candidate (U073O22D001O47)

BBa_K228005
BBa_K228005 Version 1 (Component)
AraC protein and Pbad promoter

BBa_K801100
BBa_K801100 Version 1 (Component)
RFP Coding Device - RFC10 and RFC25 compatible

BBa_K909006
BBa_K909006 Version 1 (Component)
Full-length lacZ with RBS and Terminator

BBa_K1332002
BBa_K1332002 Version 1 (Component)
Histidine tag (8 AA) and RFP (without stop codon)

BBa_K861011
BBa_K861011 Version 1 (Component)
FadD with RBS and terminator.

BBa_I732094
BBa_I732094 Version 1 (Component)
Promoter Activity Reporter (LacZ-alpha and GFP-AAV)

BBa_K1332001
BBa_K1332001 Version 1 (Component)
Histidine tag (8 AA) and RFP

BBa_K1088020
BBa_K1088020 Version 1 (Component)
lacI:LVA device (constitutive promoter, RBS and terminator)

BBa_K325210
BBa_K325210 Version 1 (Component)
Red Firefly Luciferase and LRE<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K1404002
BBa_K1404002 Version 1 (Component)
Ptac-NiCoTB, improves nickel and cobalt internalization

BBa_K775003
BBa_K775003 Version 1 (Component)
RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator

BBa_I746314
BBa_I746314 Version 1 (Component)
arabinose induced expression of PSP3 pag and mRFP

BBa_K1638016
BBa_K1638016 Version 1 (Component)
8 aa linker without BamHI restriction site and TEV recognition site

BBa_K2066547
BBa_K2066547 Version 1 (Component)
Synthetic Enhancer Project: Spacer between Promoter and RBS

BBa_K574006
BBa_K574006 Version 1 (Component)
3OC12HSL regulated by pTet and pBad

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_M39018
BBa_M39018 Version 1 (Component)
HrpL AND gate

BBa_I715052
BBa_I715052 Version 1 (Component)
Trp Leader Peptide and anti-terminator/terminator