BBa_K896000
BBa_K896000 Version 1 (Component)
SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7

BBa_K896003
BBa_K896003 Version 1 (Component)
Sulfide quinone reductase(SQR) on neutral site 2 vector

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

BBa_K748001
BBa_K748001 Version 1 (Component)
AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K113011
BBa_K113011 Version 1 (Component)
more overlapping T7 promoter

BBa_K113017
BBa_K113017 Version 1 (Component)
more overlapping T7 promoter->GFP/RFP

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

T7p
BBa_K133041 Version 1 (Component)
T7 promoter expression plasmid backbone

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

BBa_K112149
BBa_K112149 Version 1 (Component)
PmgtCB Magnesium promoter from <I>Salmonella</I>

BBa_K177007
BBa_K177007 Version 1 (Component)
PhoP/PhoQ locus from salmonella typhimurium

BBa_K294125
BBa_K294125 Version 1 (Component)
A coding sequence for one of the heat shock protein:hchA

BBa_K177031
BBa_K177031 Version 1 (Component)
mgtc testing device with phoQP from salmonella

BBa_K196007
BBa_K196007 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid) + RFP + RBS (GluColi project)

GroEL
BBa_K205000 Version 1 (Component)
GroEL is a chaperone protein that prevents polypeptide misfolding.

GroES
BBa_K205001 Version 1 (Component)
GroES is a chaperone protein that prevents polypeptide misfolding.

BBa_K177039
BBa_K177039 Version 1 (Component)
PhoP from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

BBa_J45990
BBa_J45990 Version 1 (Component)
Exponential phase expression of ATFI

BBa_K177040
BBa_K177040 Version 1 (Component)
PhoQ from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

BBa_T2025
BBa_T2025 Version 1 (Component)
Glutathione S-transferase internal domain

BBa_K174000
BBa_K174000 Version 1 (Component)
SspB proteolysis chaperone

DnaK
BBa_K205002 Version 1 (Component)
DnaK is a chaperone protein that assists with polypeptide folding

DnaJ/GrpE
BBa_K205003 Version 1 (Component)
DnaJ and GrpE are chaperone proteins that assists with polypeptide folding

BBa_K283034
BBa_K283034 Version 1 (Component)
motB (Backbone: pSB1AK3)

BBa_K283045
BBa_K283045 Version 1 (Component)
prosseA-rbs- T7 RNAP (Backbone: pSB3C5)

BBa_K283046
BBa_K283046 Version 1 (Component)
LacY (Backbone: pSB1AK3)

BBa_K189101
BBa_K189101 Version 1 (Component)
pET linkage group of GenSniper via Bottom Up approach

BBa_K189102
BBa_K189102 Version 1 (Component)
pAC linkage group of GenSniper via Bottom Up approach

BBa_K289008
BBa_K289008 Version 1 (Component)
PGBKT7(YEAST PLASMIDE BACKBONE)

BBa_K174019
BBa_K174019 Version 1 (Component)
SmtA metallothionein protein as a fusion partner

BBa_K222002
BBa_K222002 Version 1 (Component)
project of a Mid1 gene disrupted from 489 to 2021 nt

BBa_K195413
BBa_K195413 Version 1 (Component)
All in one

BBa_K357007
BBa_K357007 Version 1 (Component)
Fusion of muconolactone D-isomerase (Enzyme 3) and 3-oxoadipate enol-lactonase (Enzyme 4)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.