BBa_J56017
BBa_J56017 Version 1 (Component)
sequence containing necessary DNA for controlled chemotaxis

LTBD
BBa_K2061005 Version 1 (Component)
Heat Labile Toxin B subunit with DNA binding Domain

BBa_K385005
BBa_K385005 Version 1 (Component)
B-box sequence encoding a regulatory mRNA stem loop

BBa_K1996001
BBa_K1996001 Version 1 (Component)
EtnR1 - DNA-binding protein for EtnP in Mycobacterium NBB4

BBa_K165027
BBa_K165027 Version 1 (Component)
Kozak + Sin3 repression domain + Gli1 DNA-binding domain

BBa_K199056
BBa_K199056 Version 1 (Component)
CCAUC tRNA (9-bp Anticodon) with RFP Reporter

BBa_K606050
BBa_K606050 Version 1 (Component)
pVeg SpoVG tyrosine amber supressor tRNA double terminator

BBa_K165025
BBa_K165025 Version 1 (Component)
Kozak + Sin3 repression domain + LexA DNA-binding domain

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

LigA
BBa_K525730 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with PT7 and RBS

BBa_M31786
BBa_M31786 Version 1 (Component)
1st half of Gene III- preBamHI cut

BBa_K1381014
BBa_K1381014 Version 1 (Component)
Silencing sRNA (10 bases long) for USP45 (Based on Spot42)

BBa_K1381016
BBa_K1381016 Version 1 (Component)
Silencing sRNA (20 bases long) for USP45 (Based on Spot42)

BBa_K1905004
BBa_K1905004 Version 1 (Component)
Riboswitch to detect L1 mRNA from HPV and express BFP

BBa_K1381015
BBa_K1381015 Version 1 (Component)
Silencing sRNA (15 bases long) for USP45 (Based on Spot42)

BBa_K1905005
BBa_K1905005 Version 1 (Component)
Riboswitch to detect E6 mRNA from HPV and express YFP

BBa_K1323008
BBa_K1323008 Version 1 (Component)
sRNA control for S. epidermidis: targets the non-coding region

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243

CsgD
BBa_K1019001 Version 1 (Component)
CsgD: positive regulator of curlin genes

BBa_K648102
BBa_K648102 Version 1 (Component)
RecA with mutation of Lys 286

BBa_K199102
BBa_K199102 Version 1 (Component)
pLac-RBS-CUACC-RFP and CUACC tRNA Suppressor

BBa_K199099
BBa_K199099 Version 1 (Component)
CCAAU tRNA Suppressor and pLac-RBS-CCAAU-RFP

BBa_K1499252
BBa_K1499252 Version 1 (Component)
GFP with 2 stop codons generator + supP tRNA

BBa_K1491036
BBa_K1491036 Version 1 (Component)
Constitutive Dead ER Intein T7 RNAP with YFPH

BBa_K199101
BBa_K199101 Version 1 (Component)
pLac-RBS-CCACC-RFP and CCACC tRNA Suppressor

BBa_K2116068
BBa_K2116068 Version 1 (Component)
AND gate regulated by norR and esaR (internal esabox, overlapping with sigma 54 binding site, before

BBa_K199057
BBa_K199057 Version 1 (Component)
CCAUC tRNA (10-bp Anticodon) with RFP Reporter

BBa_K1487076
BBa_K1487076 Version 1 (Component)
eYFP Reporter for tcpI gRNA target with strong promoter.

BBa_K1487072
BBa_K1487072 Version 1 (Component)
eYFP Reporter for tcpD gRNA target with strong promoter.

BBa_K1487067
BBa_K1487067 Version 1 (Component)
eYFP Reporter for ctxA gRNA target with strong promoter.

BBa_K1487078
BBa_K1487078 Version 1 (Component)
eYFP Reporter for tcpP gRNA target with strong promoter.

BBa_K1487073
BBa_K1487073 Version 1 (Component)
eYFP Reporter for tcpE gRNA target with strong promoter.

BBa_K1487068
BBa_K1487068 Version 1 (Component)
eYFP Reporter for ctxB gRNA target with strong promoter.

BBa_K1487075
BBa_K1487075 Version 1 (Component)
eYFP Reporter for tcpH gRNA target with strong promoter.

BBa_K1487065
BBa_K1487065 Version 1 (Component)
eYFP Reporter for acfC gRNA target with strong promoter.

BBa_K1487074
BBa_K1487074 Version 1 (Component)
eYFP Reporter for tcpF gRNA target with strong promoter.

BBa_K1487071
BBa_K1487071 Version 1 (Component)
eYFP Reporter for tcpC gRNA target with strong promoter.

BBa_K1487079
BBa_K1487079 Version 1 (Component)
eYFP Reporter for tcpQ gRNA target with strong promoter.

BBa_K1487066
BBa_K1487066 Version 1 (Component)
eYFP Reporter for acfD gRNA target with strong promoter.

BBa_K1487063
BBa_K1487063 Version 1 (Component)
eYFP Reporter for acfA gRNA target with strong promoter.

BBa_K1487083
BBa_K1487083 Version 1 (Component)
eYFP Reporter for toxT gRNA target with strong promoter.

BBa_K1487070
BBa_K1487070 Version 1 (Component)
eYFP Reporter for tcpB gRNA target with strong promoter.

BBa_K1487077
BBa_K1487077 Version 1 (Component)
eYFP Reporter for tcpJ gRNA target with strong promoter.

BBa_K1487064
BBa_K1487064 Version 1 (Component)
eYFP Reporter for acfB gRNA target with strong promoter.

BBa_K1487082
BBa_K1487082 Version 1 (Component)
eYFP Reporter for tcpT gRNA target with strong promoter.

BBa_K1487069
BBa_K1487069 Version 1 (Component)
eYFP Reporter for tcpA gRNA target with strong promoter.

BBa_K1487080
BBa_K1487080 Version 1 (Component)
eYFP Reporter for tcpR gRNA target with strong promoter.

BBa_K1487081
BBa_K1487081 Version 1 (Component)
eYFP Reporter for tcpS gRNA target with strong promoter.

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.