BBa_K812012BBa_K812012 Version 1 (Component)OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
BBa_K812013BBa_K812013 Version 1 (Component)GFP-AID OsTirI polysistronic system for auxin detection in tadpole
BBa_K812014BBa_K812014 Version 1 (Component)Auxin production device for expression in tadpole
BBa_K812001BBa_K812001 Version 1 (Component)Biobricked pSC2+ plasmid with pElastase promoter for frog use
LipABBa_K836000 Version 1 (Component)lipA from B. cepacia (codon usage optimized for E. coli)
DGATBBa_K836001 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)
DGATBBa_K836002 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
lipABBa_K836003 Version 1 (Component)lipA from B. cepacia (codon usage optimized for E. coli)
gpSBBa_K836004 Version 1 (Component)Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
BBa_K836005BBa_K836005 Version 1 (Component)Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)
gpSBBa_K836006 Version 1 (Component)Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
nitRBBa_K836007 Version 1 (Component)Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Cello E. Coli PartsEco1C1G1T1_collection Version 1 (Collection)These are the Cello parts for E. Coli circuits
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Q04510+RFPBBa_K131018 Version 1 (Component)Intermediate for the Response circuit
rTT+pRBBa_K093003 Version 1 (Component)Convergent Promoter System: Forward Module: rTT+lambda pR
BBa_K101016BBa_K101016 Version 1 (Component)Dually repressed promoter with sites for TetR and P22MNT binding
BBa_K125810BBa_K125810 Version 1 (Component)slr2016 signal sequence + GFP fusion for secretion of GFP
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
pBSEP1BBa_K090401 Version 1 (Component)Gram-positive Shuttle Vector for Episomal Expression
BBa_K090403BBa_K090403 Version 1 (Component)Gram-positive Shuttle Vector for Chromosomal Integration
BBa_C0361BBa_C0361 Version 1 (Component)luxI (+LVA) - Codon-optimised for E.coli
BBa_C0378BBa_C0378 Version 1 (Component)lasI (+LVA) - Codon-optimised for E.coli
BBa_C0362BBa_C0362 Version 1 (Component)luxR - codon-optimised for E.coli
BBa_J24817BBa_J24817 Version 1 (Component)dLaRAP firefly luciferase reporter device for promoters
BBa_J24818BBa_J24818 Version 1 (Component)dLaRAP firefly luciferase reporter device for promoters
BBa_J24819BBa_J24819 Version 1 (Component)dLaRAP firefly luciferase reporter device for promoters with J23101
AD defaultBBa_K106400 Version 1 (Component)Default insert for AarI AD acceptor vectors
BBa_J24820BBa_J24820 Version 1 (Component)Renilla luciferase reporter device for DLARAP
BBa_J70031BBa_J70031 Version 1 (Component)Linker for BioScaffold Parts, example of Gamma BioScaffold part
BBa_J70032BBa_J70032 Version 1 (Component)A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)
BBa_J70033BBa_J70033 Version 1 (Component)A BioMortar Part for Creating GSGS Protein Fusions (see Design page)
BBa_J70034BBa_J70034 Version 1 (Component)A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)
BBa_J70036BBa_J70036 Version 1 (Component)A BioScaffold Part (Uses AarI) for Library Insert Preparation (see Part Design page)
BBa_J70040BBa_J70040 Version 1 (Component)A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)
BBa_J70042BBa_J70042 Version 1 (Component)6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
BBa_J70044BBa_J70044 Version 1 (Component)6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA
BBa_J70046BBa_J70046 Version 1 (Component)6his part for use with BioScaffold parts ATG-6 his-GATCC(glycine serine)-TAA
BBa_J70048BBa_J70048 Version 1 (Component)6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA
BBa_J70100BBa_J70100 Version 1 (Component)Library Vector Test Plasmid for J70040
BBa_J70110BBa_J70110 Version 1 (Component)Library Vector Test Plasmid for J70034
BBa_J70120BBa_J70120 Version 1 (Component)Library Insert Test Plasmid for J70050 and J70052
tetA(C)fBBa_J31007 Version 1 (Component)tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
mCherryBBa_J06505 Version 1 (Component)monomeric RFP optimized for bacteria
BBa_J70089BBa_J70089 Version 1 (Component)Test for part J70084
BBa_J70102BBa_J70102 Version 1 (Component)Left 6 his test part for library construction part.
BBa_J70104BBa_J70104 Version 1 (Component)Right 6 his test part for library construction part.
BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli