BBa_K615001BBa_K615001 Version 1 (Component)Zinc finger-omega subunit fusion protein
BBa_K597101BBa_K597101 Version 1 (Component)GFP-AviTag [AviTagged green fluorescent protein]
BBa_K654096BBa_K654096 Version 1 (Component)E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)
BBa_K823051BBa_K823051 Version 1 (Component)mKate2, a red monomeric fluorescent protein + RBS + Term
BBa_K748000BBa_K748000 Version 1 (Component)AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.
BBa_K748001BBa_K748001 Version 1 (Component)AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
BBa_K784015BBa_K784015 Version 1 (Component)Phage Lambda fragment 1 - head proteins
BBa_K784016BBa_K784016 Version 1 (Component)Phage Lambda fragment 2 - tail proteins
BBa_K784017BBa_K784017 Version 1 (Component)Phage Lambda fragment 3 - tail tip proteins
BBa_K784018BBa_K784018 Version 1 (Component)Phage Lambda fragment 4 - tail fiber proteins
BBa_K932001BBa_K932001 Version 1 (Component)EnvZ Protein
BBa_K784022BBa_K784022 Version 1 (Component)Phage Lambda fragment 8 - lytic proteins
BBa_K758000BBa_K758000 Version 1 (Component)UAS , this is the enhancer . And this enhancer is used to identify the protein localization .
BBa_K758002BBa_K758002 Version 1 (Component)GAL4, this protein binds the UAS
BBa_K763005BBa_K763005 Version 1 (Component)pADH2 + Gene encoding YAP1 protein
BBa_K758004BBa_K758004 Version 1 (Component)GAL4, this part coding protein binds and activates the UAS sequence
UAS-EGFPBBa_K758006 Version 1 (Component)UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.
BBa_K812012BBa_K812012 Version 1 (Component)OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
BBa_K737016BBa_K737016 Version 1 (Component)This part conteins gvpA protein's coding sequence.
BBa_K737017BBa_K737017 Version 1 (Component)This part conteins gvpC-20psi protein
BBa_K737011BBa_K737011 Version 1 (Component)gvpC-GFP fusion protein with medium constitutive promoter
BBa_K737021BBa_K737021 Version 1 (Component)gvpC-20 coding gene with GFP mut 3.1 fusion gene
BBa_K737012BBa_K737012 Version 1 (Component)gvpC-GFP fusion protein UH with medium constitutive promoter
BBa_K737022BBa_K737022 Version 1 (Component)This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene
BBa_K737013BBa_K737013 Version 1 (Component)gvpC-GFP fusion protein
BBa_K737014BBa_K737014 Version 1 (Component)gvpC-GFP fusion protein UH
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
BBa_K873000BBa_K873000 Version 1 (Component)heat shock 10kDa protein(hsp 10) groES coding resquence
BBa_K739004BBa_K739004 Version 1 (Component)Ax21 protein - signaling part
pLac ColE2BBa_K914001 Version 1 (Component)pLac Colicin E2 Immunity protein
BBa_K914002BBa_K914002 Version 1 (Component)repressilator RBS Colicin E2 Immunity protein
BBa_K883160BBa_K883160 Version 1 (Component)CCMV_Negative coat protein
BBa_K883151BBa_K883151 Version 1 (Component)CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor
gpSBBa_K836006 Version 1 (Component)Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
linkerBBa_K157013 Version 1 (Component)15 aa flexible glycine-serine protein domain linker; Freiburg standard
BBa_K156009BBa_K156009 Version 1 (Component)OFP (orange fluorescent protein)
SBFP2BBa_K156010 Version 1 (Component)SBFP2 (strongly enhanced blue fluorescent protein)
BBa_K125810BBa_K125810 Version 1 (Component)slr2016 signal sequence + GFP fusion for secretion of GFP
BBa_I0466BBa_I0466 Version 1 (Component)RhlR Protein Generator
BBa_K105000BBa_K105000 Version 1 (Component)pSB1A2_fusion
BBa_K116001BBa_K116001 Version 1 (Component)nhaA promoter, that can be regulated by pH and nhaR protein.
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_K079052BBa_K079052 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
BBa_K127013BBa_K127013 Version 1 (Component)gas vesicle protein A from cyanobacteria Anabaena