Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ETH Zurich iGEM Team 2005
Date created: 2005-09-21 11:00:00
Date modified: 2015-08-31 04:08:15
Sensing Device B (cI+LVA) TESTER (Pr+GFP+Prm+RFP)
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J05504_sequence (Version 1) |
Description
A debug version of the event sensing and processing system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI+LVA (degradation tag) production dependent on an external event. It works somehow like an inverter:STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream the Pr promoter will be constitutively active, producing GFP (green fluorescence), while Prm+ will have low or no activity (no/low RFP - red fluorescence).
STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream the Pr promoter will be repressed (no GFP, i.e. no green fluorescence) by cI, while Prm+ will be activated, producing RFP (red fluorescence).
Source
Registry 7.05| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public public public public | BBa_R0040 BBa_B0034 BBa_C0012 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0034 BBa_C0051 BBa_B0012 BBa_B0011 BBa_R0051 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012 BBa_I12007 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012 | p(tetR) BBa_B0034 lacI BBa_B0010 BBa_B0012 lacI+pL BBa_B0034 cI lam BBa_B0012 BBa_B0011 cI lam BBa_B0034 GFP BBa_B0010 BBa_B0012 Prm + BBa_B0034 mRFP1 BBa_B0010 BBa_B0012 |