BBa_K782053
BBa_K782053 Version 1 (Component)
XbaI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782054
BBa_K782054 Version 1 (Component)
XbaI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782055
BBa_K782055 Version 1 (Component)
XbaI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782056
BBa_K782056 Version 1 (Component)
SacI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782057
BBa_K782057 Version 1 (Component)
SacI/SpeI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782058
BBa_K782058 Version 1 (Component)
SacI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K1329002
BBa_K1329002 Version 1 (Component)
Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin

BBa_K1362106
BBa_K1362106 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct with additional C-teminal insertion site

BBa_K1362107
BBa_K1362107 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

Stop
BBa_K1362473 Version 1 (Component)
C terminal Stop insert for RFC[???] N intein constructs with two insertion sites

BBa_K1362474
BBa_K1362474 Version 1 (Component)
N terminal Gly insert for RFC[???] C intein constructs with two insertion sites

BBa_K1648006
BBa_K1648006 Version 1 (Component)
Insertion kit with GFP-nanobody

BBa_K2027010
BBa_K2027010 Version 1 (Component)
Repetitive Insertion of 4D Melanin-Binding Heptapeptide

BBa_K1962003
BBa_K1962003 Version 1 (Component)
Colicin Ia::Ssp2 Chimera

BBa_K1962007
BBa_K1962007 Version 1 (Component)
Colicin E9::Ssp1 Chimera

BBa_K1962008
BBa_K1962008 Version 1 (Component)
Colicin E9::Ssp2 Chimera

BBa_K1987000
BBa_K1987000 Version 1 (Component)
GFP with BsaI sites for promoter insertion

dCas9 B
BBa_K1903002 Version 1 (Component)
dCas9 with protein insertion frames version B

dCas9 A
BBa_K1903001 Version 1 (Component)
dCas9 with protein insertion frames version A

BBa_K1903022
BBa_K1903022 Version 1 (Component)
Insertion 1 for dCas9 version B

BBa_K1903023
BBa_K1903023 Version 1 (Component)
Insertion 2 for dCas9 version B

Insertion
BBa_K1903020 Version 1 (Component)
Insertion 1 for dCas9 version A

Insertion
BBa_K1903021 Version 1 (Component)
Insertion 2 for dCas9 version A

BBa_J133650
BBa_J133650 Version 1 (Component)
Chimerabrick Replacement Cassette

BBa_J119009
BBa_J119009 Version 1 (Component)
pLacI-RBS-T7RNAP with sites for oligo insertion

pINDEL
BBa_K551001 Version 1 (Component)
plasmid of insertion and deletion

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o