lytCFLAG
BBa_K733008 Version 1 (Component)
<i>lytC</i> + linker + FLAG???

BBa_K784016
BBa_K784016 Version 1 (Component)
Phage Lambda fragment 2 - tail proteins

BBa_K784017
BBa_K784017 Version 1 (Component)
Phage Lambda fragment 3 - tail tip proteins

BBa_K784018
BBa_K784018 Version 1 (Component)
Phage Lambda fragment 4 - tail fiber proteins

BBa_K932001
BBa_K932001 Version 1 (Component)
EnvZ Protein

BBa_K784022
BBa_K784022 Version 1 (Component)
Phage Lambda fragment 8 - lytic proteins

BBa_K758000
BBa_K758000 Version 1 (Component)
UAS , this is the enhancer . And this enhancer is used to identify the protein localization .

BBa_K758002
BBa_K758002 Version 1 (Component)
GAL4, this protein binds the UAS

BBa_K763005
BBa_K763005 Version 1 (Component)
pADH2 + Gene encoding YAP1 protein

BBa_K784015
BBa_K784015 Version 1 (Component)
Phage Lambda fragment 1 - head proteins

GFP-AID
BBa_K812010 Version 1 (Component)
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

CBDcex
BBa_K863101 Version 1 (Component)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K873000
BBa_K873000 Version 1 (Component)
heat shock 10kDa protein(hsp 10) groES coding resquence

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

BBa_K874020
BBa_K874020 Version 1 (Component)
Myc-Linker (inc. start codon)

BBa_K769000
BBa_K769000 Version 1 (Component)
Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator

BBa_K769004
BBa_K769004 Version 1 (Component)
NpSRII-9a.a.linker_NpHtrII-EnvZ

BBa_K769003
BBa_K769003 Version 1 (Component)
Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator

BBa_K782004
BBa_K782004 Version 1 (Component)
TALA:NLS DNA binding domain

BBa_K782005
BBa_K782005 Version 1 (Component)
TALD:NLS DNA binding domain

BBa_K782006
BBa_K782006 Version 1 (Component)
TALB:NLS DNA binding domain

BBa_K782007
BBa_K782007 Version 1 (Component)
NicTAL12:NLS DNA binding domain

BBa_K769002
BBa_K769002 Version 1 (Component)
Kinase domain of EnvZ

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_K883160
BBa_K883160 Version 1 (Component)
CCMV_Negative coat protein

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Tm-Fluo-li
BBa_K157014 Version 1 (Component)
Tm-Fluo-linker-cYFP

Tm-Fluolin
BBa_K157016 Version 1 (Component)
Tm-Fluolinker-cCFP

linker
BBa_K157009 Version 1 (Component)
Split fluorophore linker; Freiburg standard

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

BBa_K157012
BBa_K157012 Version 1 (Component)
Strep affinity tag II; Freiburg standard

SP-AntiNIP
BBa_K157022 Version 1 (Component)
SP-AntiNIP-GGGSlinker