Cas9Cas9 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubi H99
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
GFPGFP Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S
HYGHygromicin_Resistance Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
mCherrymCherry Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S strain
amdSamdS Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
APP1GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
ACT1GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
BBa_J61101BBa_J61101 Version 1 (Component)Ribosome Binding Site Family Member
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
SEGA021SEGA021 Version 1 (Component)natural linear DNA.
SEGA022SEGA022 Version 1 (Component)natural linear DNA.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_J61120BBa_J61120 Version 1 (Component)Ribosome Binding Site Family Member
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
Amp CasBBa_I13800 Version 1 (Component)Ampicillin Resistance Cassette
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_J61100BBa_J61100 Version 1 (Component)Ribosome Binding Site Family Member
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_K1321340BBa_K1321340 Version 1 (Component)Double CBD (dCBD) with N-terminal linker
BBa_J56012BBa_J56012 Version 1 (Component)Invertible sequence of dna includes Ptrc promoter
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
PBS_F24PBS_F24 Version 1 (Component) BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
RBS-spoVGBBa_K143021 Version 1 (Component)SpoVG ribosome binding site (RBS) for B. subtilis
BBa_K1179017BBa_K1179017 Version 1 (Component)A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation
rhIIBBa_C0070 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
mCherryBBa_J06504 Version 1 (Component)monomeric RFP optimized for bacteria
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A