Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

AraC
BBa_R0080 Version 1 (Component)
Promoter (AraC regulated)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_K228000
BBa_K228000 Version 1 (Component)
T7ptag(T7polymerase with amber mutation)

BBa_K228001
BBa_K228001 Version 1 (Component)
SupD-tRNA

BBa_K228876
BBa_K228876 Version 1 (Component)
AND GATE AraC+RBS(J44001)+T7ptag+Sal+SupD

Ara
BBa_K808000 Version 1 (Component)
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit

BBa_K228260
BBa_K228260 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(J44001)+T7ptag

BBa_K228009
BBa_K228009 Version 1 (Component)
AraC protein(reversed sequence) and Pbad promoter

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K228852
BBa_K228852 Version 1 (Component)
AND GATE Sal+SupD+LacP+RBS(J44001)+T7ptag

BBa_K228118
BBa_K228118 Version 1 (Component)
AND GATE AraC + SupD + LacP +RBS(J44001)+T7ptag

BBa_K228043
BBa_K228043 Version 1 (Component)
AND Gate LuxR-rbs(J44001)-T7ptag-AraC-SupD

BBa_K228823
BBa_K228823 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J44001)+T7ptag

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K228877
BBa_K228877 Version 1 (Component)
AND GATE AraC+RBS(B0033)+T7ptag+Sal+SupD

BBa_K228870
BBa_K228870 Version 1 (Component)
AND GATE AraC+RBS(B0030)+T7ptag+Sal+SupD

BBa_K228874
BBa_K228874 Version 1 (Component)
AND GATE AraC+RBS(J61107)+T7ptag+Sal+SupD

BBa_K228871
BBa_K228871 Version 1 (Component)
AND GATE AraC+RBS(B0032)+T7ptag+Sal+SupD

BBa_K228872
BBa_K228872 Version 1 (Component)
AND GATE AraC+RBS(B0034)+T7ptag+Sal+SupD

BBa_K228873
BBa_K228873 Version 1 (Component)
AND GATE AraC+RBS(J61100)+T7ptag+Sal+SupD

BBa_K228878
BBa_K228878 Version 1 (Component)
AND GATE AraC+RBS(B0031)+T7ptag+Sal+SupD

BBa_K228851
BBa_K228851 Version 1 (Component)
Sal+supD+terminator

BBa_K228875
BBa_K228875 Version 1 (Component)
AND GATE AraC+RBS(J61127)+T7ptag+Sal+SupD

BBa_K228255
BBa_K228255 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0030)+T7ptag

BBa_K228258
BBa_K228258 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag

BBa_K228263
BBa_K228263 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(J61127)+T7ptag

BBa_K228257
BBa_K228257 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0032)+T7ptag

BBa_K228256
BBa_K228256 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0031)+T7ptag