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Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

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Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Public

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

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tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

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BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

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cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

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Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

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eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

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luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

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BBa_I716210
BBa_I716210 Version 1 (Component)
BamHI endonuclease without start codon

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BBa_K809601
BBa_K809601 Version 1 (Component)
GAL promoter (kozak) without start codon ATG

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BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

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BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

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BBa_K112105
BBa_K112105 Version 1 (Component)
BamHI endonuclease without start or stop codons

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ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

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BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

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BBa_K112108
BBa_K112108 Version 1 (Component)
BglII endonuclease without start or stop codons

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RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

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Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

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BBa_K112104
BBa_K112104 Version 1 (Component)
BamHI endonuclease without stop codon

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BBa_K112113
BBa_K112113 Version 1 (Component)
Ligase without start or stop codons

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His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

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dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

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cre
BBa_K112102 Version 1 (Component)
Cre enzyme without start or stop codons

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RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

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RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

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BBa_K112107
BBa_K112107 Version 1 (Component)
BglII endonuclease without stop codon

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BBa_I766217
BBa_I766217 Version 1 (Component)
Stop-D-BamHI-Adh Terminator Adaptor

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BBa_M36117
BBa_M36117 Version 1 (Component)
Stop Codon

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Adapter
BBa_I732007 Version 1 (Component)
BglII + BamHI

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BBa_K1332002
BBa_K1332002 Version 1 (Component)
Histidine tag (8 AA) and RFP (without stop codon)

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BBa_I746916
BBa_I746916 Version 1 (Component)
superfolder GFP coding sequence

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BBa_K1051004
BBa_K1051004 Version 1 (Component)
Stop codon free mOrange in RFC[23] standard

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BBa_K1582030
BBa_K1582030 Version 1 (Component)
mTagBFP without stop codon

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BBa_K1582037
BBa_K1582037 Version 1 (Component)
LC without stop codon

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BBa_K1582039
BBa_K1582039 Version 1 (Component)
Thc_Cut1 without stop codon

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BBa_K1582038
BBa_K1582038 Version 1 (Component)
FsC without stop codon

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aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

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BBa_K1051053
BBa_K1051053 Version 1 (Component)
K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 terminator)

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BBa_K2066518
BBa_K2066518 Version 1 (Component)
mCherry (with start codon)

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lacZ-alpha
BBa_I732006 Version 1 (Component)
lacZ alpha fragment

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BBa_I766340
BBa_I766340 Version 1 (Component)
stop-bamHI

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STOP
BBa_J176016 Version 1 (Component)
STOP

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BBa_K1051009
BBa_K1051009 Version 1 (Component)
e.coli terminator ilvGEDA_T with stop codon

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BBa_K1583059
BBa_K1583059 Version 1 (Component)
His-tag without start codon

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BBa_K1051002
BBa_K1051002 Version 1 (Component)
Stop codon free EYFP in RFC[23] standard

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BBa_K1601000
BBa_K1601000 Version 1 (Component)
Cytochrome C S.oneidensis without stop codon

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BBa_K1638016
BBa_K1638016 Version 1 (Component)
8 aa linker without BamHI restriction site and TEV recognition site

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BBa_K112112
BBa_K112112 Version 1 (Component)
Ligase with a start codon without a stop codon

Public

Stop
BBa_K1362437 Version 1 (Component)
Tripple A

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