Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_B0012BBa_B0012 Version 1 (Component)TE from coliphageT7
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
luxrBBa_C0062 Version 1 (Component)luxR repressor/activator, (no LVA?)
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
BBa_J61101BBa_J61101 Version 1 (Component)Ribosome Binding Site Family Member
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
BBa_J61120BBa_J61120 Version 1 (Component)Ribosome Binding Site Family Member
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
BBa_J61100BBa_J61100 Version 1 (Component)Ribosome Binding Site Family Member
lasRBBa_C0179 Version 1 (Component)lasR activator from P. aeruginosa PAO1(no LVA)
BBa_J85600BBa_J85600 Version 1 (Component)attB1 recombination site
luxIBBa_C0161 Version 1 (Component)autoinducer synthetase for AHL (no LVA)
BBa_K2044000BBa_K2044000 Version 1 (Component)Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8
BBa_F2620BBa_F2620 Version 1 (Component)3OC<sub>6</sub>HSL -> PoPS Receiver
BBa_J52008BBa_J52008 Version 1 (Component)luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
rhIRBBa_C0171 Version 1 (Component)rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
BBa_K2044001BBa_K2044001 Version 1 (Component)Based on our project, <2-4-8> is a feasible pathway from Site No. 2 to Site No. 8
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_I15008BBa_I15008 Version 1 (Component)heme oxygenase (ho1) from Synechocystis
FurBO_11498 Version 1 (Component) BBa_K2044012BBa_K2044012 Version 1 (Component)Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.
lasIBBa_C0178 Version 1 (Component)autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
BBa_K2044005BBa_K2044005 Version 1 (Component)Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.