NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

BBa_K864150
BBa_K864150 Version 1 (Component)
Cat-SacB cassette for scarless chromosomal knockout

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

BBa_K314100
BBa_K314100 Version 1 (Component)
High constitutive expression cassette

kanR
BBa_P1003 Version 1 (Component)
kanamycin resistance cassette

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K1520504
BBa_K1520504 Version 1 (Component)
Rbs-luxI(scarless)

BBa_I739001
BBa_I739001 Version 1 (Component)
Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

BBa_K1520505
BBa_K1520505 Version 1 (Component)
Rbs-tetR(scarless)

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_J23008
BBa_J23008 Version 1 (Component)
[key3c] riboregulator for lock3 variants

BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

BBa_K1520502
BBa_K1520502 Version 1 (Component)
Rbs-lacI(scarless)

CAT
BBa_C0100 Version 1 (Component)
Enzyme, chloramphenicol acetyltransferase or CAT

BBa_K1520501
BBa_K1520501 Version 1 (Component)
Rbs-luxR(scarless)

BBa_K541501
BBa_K541501 Version 1 (Component)
Promoter Pveg, RBS spoVG and SacB signal peptide for B. subtilis

CmR
BBa_J61000 Version 1 (Component)
chloramphenicol resistance cassette

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

cmR
BBa_P1004 Version 1 (Component)
chloramphenicol resistance cassette

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

BBa_K322922
BBa_K322922 Version 1 (Component)
cat + sacB for BRIDGE: A biobrick compatible markerless recombineering method

BBa_J70304
BBa_J70304 Version 1 (Component)
constitutive cat cassette for Mesoplasma florum