NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

TAT-trojan
BBa_K792003 Version 1 (Component)
Import enhancer - HIV TAT penetratin (trojan peptide)

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

Polyargini
BBa_K792004 Version 1 (Component)
Import enhancer - Polyarginine (trojan peptide)

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

CD4 signal
BBa_I712009 Version 1 (Component)
CD4 signal peptide; localizes to plasma membrane

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_K792009
BBa_K792009 Version 1 (Component)
Yeast exportable His-rich peptide w/enhanced import (1)

BBa_K380000
BBa_K380000 Version 1 (Component)
TAT cell-penetrating peptide

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K817021
BBa_K817021 Version 1 (Component)
RBS-SP1-Penetratin-Flag

BBa_K380001
BBa_K380001 Version 1 (Component)
N-part TAT cell-penetrating peptide

BBa_K792011
BBa_K792011 Version 1 (Component)
Yeast exportable His-rich peptide w/enhanced import (2)

BBa_E2030
BBa_E2030 Version 1 (Component)
enhanced version of EYFP, yeast-optimized

eGFP+His
BBa_K193202 Version 1 (Component)
EGFP(enhanced Green Fluorescent Protein) with His tag

BBa_K817022
BBa_K817022 Version 1 (Component)
RBS-SP2-Penetratin-Flag

BBa_K817023
BBa_K817023 Version 1 (Component)
RBS-SP3-Penetratin-Flag

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_I712015
BBa_I712015 Version 1 (Component)
HIV-1 protease cleavage site

BBa_J36835
BBa_J36835 Version 1 (Component)
Lipoprotein signal peptide

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

BBa_K817024
BBa_K817024 Version 1 (Component)
RBS-SP1-Penetratin-Flag-xx

PEST
BBa_J176018 Version 1 (Component)
PEST

pLux/Tet
BBa_K176000 Version 1 (Component)
pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

HisTag
BBa_K792005 Version 1 (Component)
Histidine rich peptide (His Tag)

BBa_K380002
BBa_K380002 Version 1 (Component)
LMWP cell-penetrating peptide

BBa_K380004
BBa_K380004 Version 1 (Component)
Tp10 cell-penetrating peptide

ECFP
BBa_E0024 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP