Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

mRFPr
BBa_J31008 Version 1 (Component)
RFP reverse

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

BBa_K199118
BBa_K199118 Version 1 (Component)
T7 promoter + RBS + RFP

BBa_K1491009
BBa_K1491009 Version 1 (Component)
RBS-mRFP-His

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

mRFP
BBa_K1615089 Version 1 (Component)
mRFP RFC25

mRFP1
BBa_E2050 Version 1 (Component)
derivative of mRFP1, yeast-optimized

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

BBa_I13521
BBa_I13521 Version 1 (Component)
Ptet mRFP

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites

eyfp
BBa_E0031 Version 1 (Component)
EYFP (RBS+ LVA-)

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

BBa_I715039
BBa_I715039 Version 1 (Component)
pLac-RBS-RFP

BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

mRFP1
BBa_J04650 Version 1 (Component)
E1010.B0015

RBS-His-Sc
BBa_K1602031 Version 1 (Component)
HisTag-Scaffold_Si4-Tag fusion protein with RBS BBa_B0034