SynBioHub

Public

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Public

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

Public

SpeI
SpeI Version 1 (Component)

Public

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

Public

SX scar
BBa_G0002 Version 1 (Component)
SpeI/XbaI mixed site

Public

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

Public

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

Public

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

Public

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

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BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

Public

pSC101
BBa_I50041 Version 1 (Component)
pSC101 origin of replication, reverse

Public

scar
BBa_G0001 Version 1 (Component)
XbaI/SpeI mixed site, reverse

Public

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

Public

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

Public

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

Public

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

Public

BBa_J85600
BBa_J85600 Version 1 (Component)
attB1 recombination site

Public

scar
BBa_G0004 Version 1 (Component)
SpeI/NheI mixed site

Public

scar
BBa_G0003 Version 1 (Component)
NheI/XbaI mixed site

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BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030

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mRFPr
BBa_J31008 Version 1 (Component)
RFP reverse

Public

BBa_K299107
BBa_K299107 Version 1 (Component)
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)

Public

AvrII/XbaI
BBa_B0104 Version 1 (Component)
AvrII/XbaI mixed site

Public

NheI
BBa_B0045 Version 1 (Component)
NheI restriction enzyme site (XbaI, SpeI compatible overhang)

Public

scar
BBa_G0005 Version 1 (Component)
NheI/SpeI mixed site

Public

scar
BBa_G0006 Version 1 (Component)
XbaI/NheI mixed site

Public

RFC 37
BBa_K245080 Version 1 (Component)
scar Xba/Spe NgoMIV

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BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

Public

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

Public

BBa_K1088021
BBa_K1088021 Version 1 (Component)
Mixed site (ACTAGA)

Public

AvrII
BBa_B0048 Version 1 (Component)
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)

Public

BBa_J44002
BBa_J44002 Version 1 (Component)
pBAD reverse

Public

scar
BBa_K300994 Version 1 (Component)
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev

Public

VR
BBa_G00102 Version 1 (Component)
Reverse BioBrick primer annealing site (VR binding site)

Public

scar
BBa_K300995 Version 1 (Component)
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev

Public

BBa_K228009
BBa_K228009 Version 1 (Component)
AraC protein(reversed sequence) and Pbad promoter

Public

BBa_K300996
BBa_K300996 Version 1 (Component)
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix)

Public

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

Public

BBa_K886000
BBa_K886000 Version 1 (Component)
Fixed lox71

Public

BBa_K300997
BBa_K300997 Version 1 (Component)
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix)

Public

difR
BBa_I742102 Version 1 (Component)
dif site with reverse orientation

Public

BBa_K228004
BBa_K228004 Version 1 (Component)
NahR( reverse) - salicylate promoter

Public

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

Public

I-SceI RS
BBa_K175027 Version 1 (Component)
I-SceI restriction site

Public

TOPO
BBa_B0044 Version 1 (Component)
Topoisomerase I mediated cloning site (reverse)

Public

scar
BBa_J107060 Version 1 (Component)
XbaI/NheI mixed site without the initial CT nucleotides

Public

BBa_I13457
BBa_I13457 Version 1 (Component)
Improved insertion site with two RNAse E cut sites

Public

SpeI
BBa_M31992 Version 1 (Component)
SpeI restriction enzyme site

Public

BBa_K1813019
BBa_K1813019 Version 1 (Component)
LacI Repressor Reversed

Public

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

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