ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

cinr
BBa_C0077 Version 1 (Component)
cinR activator from Rhizobium leguminosarum (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

rhlR
BBa_C0071 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)

rhIR
BBa_C0171 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

cinR
BBa_R0078 Version 1 (Component)
Promoter (cinR and HSL regulated)

BBa_I746352
BBa_I746352 Version 1 (Component)
delta activator from phiR73 phage

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

cinR
BBa_R0077 Version 1 (Component)
Promoter (cinR and HSL regulated, RBS+)

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_I746350
BBa_I746350 Version 1 (Component)
ogr activator from P2 phage

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

eyfp
BBa_E0031 Version 1 (Component)
EYFP (RBS+ LVA-)

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

penI
BBa_C0074 Version 1 (Component)
penI repressor from Bacillus licheniformis (+LVA)

cinI
BBa_C0076 Version 1 (Component)
autoinducer synthetase

cinR
BBa_C0177 Version 1 (Component)
cinR (-LVA)

rhiI
BBa_K594005 Version 1 (Component)
a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57

ecfp
BBa_E0021 Version 1 (Component)
ECFP (RBS+ LVA-)

lasI
BBa_C0178 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

BBa_K082003
BBa_K082003 Version 1 (Component)
GFP(+LVA)

BBa_K773003
BBa_K773003 Version 1 (Component)
mCherry-LVA