NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

pSB1AT3
pSB1AT3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

pSB1AK3
pSB1AK3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

pSB1A2
pSB1A2 Version 1 (Component)
pSB1A2 (Replaced by pSB1A3)

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

pLac
BBa_K1638000 Version 1 (Component)
Lac operator

pSB1K3
pSB1K3 Version 1 (Component)
High copy BioBrick assembly plasmid

pSB1AK8
pSB1AK8 Version 1 (Component)
Eukaryotic terminator vector (formerly BBa_J52017)

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

Screening
pSB1A10 Version 1 (Component)
Screening Plasmid v1.0

pSB1AC3
pSB1AC3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

pSB1A3
pSB1A3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_K199049
BBa_K199049 Version 1 (Component)
pLac+RBS+CAT+TT on pSB1AK3

pSB3K3
pSB3K3 Version 1 (Component)
Low to medium copy BioBrick standard vector

pSB4A3
pSB4A3 Version 1 (Component)
pSB4A3 (Replaced by pSB4A5)

pSB2K3
pSB2K3 Version 1 (Component)
Inducible copy number BioBrick plasmid

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

BBa_I715039
BBa_I715039 Version 1 (Component)
pLac-RBS-RFP

pSB1A1
pSB1A1 Version 1 (Component)
pUC19 with a BioBrick cloning site (Replaced by pSB1A3)

pSB1AK3-1
pSB1AK3_1 Version 1 (Component)
PSB1AK3 with ccdB insert

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_S04224
BBa_S04224 Version 1 (Component)
CAT+TT on pSB1AK3

pSB1AC0
pSB1AC0 Version 1 (Component)
pSB1AC0

BBa_J33207
BBa_J33207 Version 1 (Component)
lac promoter and lacZ

BBa_K199116
BBa_K199116 Version 1 (Component)
RBS-RFP-TT-pLac-RBS-LuxR-TT

pSB1A7
pSB1A7 Version 1 (Component)
Transcriptionally insulated high copy BioBrick plasmid

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)