BBa_K581015
BBa_K581015 Version 1 (Component)
pBAD-supD-plux_inv-T7ptag

BBa_K764020
BBa_K764020 Version 1 (Component)
Inducible pBad/araC promoter + BBa_B0034

BBa_J72179
BBa_J72179 Version 1 (Component)
AraC-pBad

BBa_K737037
BBa_K737037 Version 1 (Component)
J23106,P0412,R0011,ClaI site,SalI site,truncated Spot42

BBa_K914000
BBa_K914000 Version 1 (Component)
pLac-supD-T

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

BBa_K113007
BBa_K113007 Version 1 (Component)
pBad/araC->T7 polymerase

BBa_K113009
BBa_K113009 Version 1 (Component)
pBad/araC

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K136066
BBa_K136066 Version 1 (Component)
araC/pBad - EnvZ*

BBa_K112149
BBa_K112149 Version 1 (Component)
PmgtCB Magnesium promoter from <I>Salmonella</I>

BBa_K177007
BBa_K177007 Version 1 (Component)
PhoP/PhoQ locus from salmonella typhimurium

BBa_K177025
BBa_K177025 Version 1 (Component)
GFP under araC promotor

AraC O2
BBa_R0081 Version 1 (Component)
Inhibitor (AraC loop attachment with O2 site)

BBa_K233003
BBa_K233003 Version 1 (Component)
AND Gate 1

BBa_K233005
BBa_K233005 Version 1 (Component)
AND gate 2

BBa_K233006
BBa_K233006 Version 1 (Component)
AND Gate 3

BBa_K177031
BBa_K177031 Version 1 (Component)
mgtc testing device with phoQP from salmonella

BBa_K091223
BBa_K091223 Version 1 (Component)
AND Gate supporting construct

BBa_K228000
BBa_K228000 Version 1 (Component)
T7ptag(T7polymerase with amber mutation)

BBa_K228001
BBa_K228001 Version 1 (Component)
SupD-tRNA

BBa_K259005
BBa_K259005 Version 1 (Component)
AraC Rheostat Promoter

BBa_K228801
BBa_K228801 Version 1 (Component)
RBS(B0030)+lacI(C0012)

BBa_K228807
BBa_K228807 Version 1 (Component)
RBS(B0030)+tetR(C0040)

BBa_K228101
BBa_K228101 Version 1 (Component)
LacP(R0010) + RBS(B0030) + T7ptag(K228000) + terminator(B0015)

BBa_K228822
BBa_K228822 Version 1 (Component)
lacP(R0010)+SupD(K228001)+terminator(B0015)

BBa_K228100
BBa_K228100 Version 1 (Component)
SupD + terminator

BBa_K228008
BBa_K228008 Version 1 (Component)
AraC regulatory protein

BBa_K190037
BBa_K190037 Version 1 (Component)
GVP with pBad/araC promoter

BBa_K228009
BBa_K228009 Version 1 (Component)
AraC protein(reversed sequence) and Pbad promoter

BBa_K228005
BBa_K228005 Version 1 (Component)
AraC protein and Pbad promoter

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_K235028
BBa_K235028 Version 1 (Component)
[K235024][K235001] (NAND gate control test, positive control)

BBa_K228823
BBa_K228823 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J44001)+T7ptag

BBa_K228200
BBa_K228200 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228201
BBa_K228201 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228202
BBa_K228202 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228203
BBa_K228203 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228204
BBa_K228204 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228205
BBa_K228205 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228206
BBa_K228206 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228207
BBa_K228207 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K228208
BBa_K228208 Version 1 (Component)
RBS->T7ptag->Terminator

BBa_K177039
BBa_K177039 Version 1 (Component)
PhoP from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2