BBa_K1179008BBa_K1179008 Version 1 (Component)A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation
BBa_M333334BBa_M333334 Version 1 (Component)Produces Beta-carotene from the biochemical input GGPP
BBa_K1642009BBa_K1642009 Version 1 (Component)Expression of GFP under the control of Pdark
BBa_J04606BBa_J04606 Version 1 (Component)Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)
BBa_K323041BBa_K323041 Version 1 (Component)ATG cYFP link HIVC
BBa_K323068BBa_K323068 Version 1 (Component)Blues link nCFP stop
BBa_K323073BBa_K323073 Version 1 (Component)PBSII link nCFP stop
2gen:ArsRBBa_K935004 Version 1 (Component)E. coli ArsR generator coupled to pArs-ArsR-RFP
BBa_E3030BBa_E3030 Version 1 (Component)FRET acceptor YPet (optimized to work with CyPet (Cyan FP) as donor
BBa_K2057005BBa_K2057005 Version 1 (Component)Expression of gfp under the regulation of promoter ChiA74
BBa_K1510931BBa_K1510931 Version 1 (Component)Sequence integrated the luxR gene and pluxR promoter together.
BBa_K1962013BBa_K1962013 Version 1 (Component)A pH Sensing Device Based on The GadA Promoter
BBa_K1962014BBa_K1962014 Version 1 (Component)A pH Sensing Device Based on The Asr Promoter
BBa_K549005BBa_K549005 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_K831007BBa_K831007 Version 1 (Component)Inducible MqsR toxin under the control of tetR promoter
BBa_K831006BBa_K831006 Version 1 (Component)Inducible MqsR toxin under the control of tetR promoter
BBa_K2117010BBa_K2117010 Version 1 (Component)eYFP expressed by the constitutive Y. lipolytica promoter TEF1
BBa_K1465105BBa_K1465105 Version 1 (Component)Fumarate reductase (Fum) under control of the T7 promoter
BBa_K831015BBa_K831015 Version 1 (Component)Inducible HipA7 toxin under the control of prm promoter
BBa_K091210BBa_K091210 Version 1 (Component)lacIQ1 promoter under the control of LacI repressor
BBa_K091211BBa_K091211 Version 1 (Component)lacIQ promoter under the control of LacI_I12 repressor
BBa_K1642011BBa_K1642011 Version 1 (Component)Expression of Halorhodopsin under the control of Pdrak
PcpcG2-HRBBa_K1642010 Version 1 (Component)Expression of Halorhodopsin under the control of PcpcG2
BBa_I3600BBa_I3600 Version 1 (Component)Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)
PfecBBa_K257000 Version 1 (Component)fec promoter (Pfec) - inducible through the Fec transduction pathway (ferric citrate)
BBa_K1951011BBa_K1951011 Version 1 (Component)desABCD cluster producing biosynthesis pathway to make Desferrioxamine B
BBa_K656008BBa_K656008 Version 1 (Component)Yellow fluorescence protein bound to dockerin from C. cellulolyticum
BBa_K656007BBa_K656007 Version 1 (Component)Cyan fluorescent protein bound to dockerin of C. thermocellum
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1216007BBa_K1216007 Version 1 (Component)Variant of the wild-type pLuxR promoter with lower sensitivity
BBa_K1933103BBa_K1933103 Version 1 (Component)constitutive expression of CBDclos fused to BclA with 6xHis tag
BBa_K1933100BBa_K1933100 Version 1 (Component)constitutive expression of CBDcex fused to INPNC with 6xHis tag
BBa_K1933102BBa_K1933102 Version 1 (Component)constitutive expression of CBDcex fused to BclA with 6xHis tag
BBa_K1933101BBa_K1933101 Version 1 (Component)constitutive expression of CBDclos fused to INPNC with 6xHis tag
BBa_K1621003BBa_K1621003 Version 1 (Component)TeNT_Hc - C-terminal part of the heavy chain (tetanus toxin)
BBa_J100242BBa_J100242 Version 1 (Component)mdtG Promotor in the Marbox Regulon Induced by Paraquat/Asprin
BBa_K2110015BBa_K2110015 Version 1 (Component)The key gene for accumulation of PHA in P.putida KT2440
BBa_K1316016BBa_K1316016 Version 1 (Component)constitutively expressed eGFP under the control of J23110 Anderson promoter
BBa_K2110016BBa_K2110016 Version 1 (Component)The key gene for utilization of ethylene glycolin P.putida KT2440.
BBa_K1351022BBa_K1351022 Version 1 (Component)FsrA: Fur-regulated sRNA which binds the binding site sdhC
FecR 1-85BBa_K257020 Version 1 (Component)FecR 1-85 - amplification of the ferric citrate induction response
BBa_K143003BBa_K143003 Version 1 (Component)5??? Integration Sequence for the PyrD locus of B. subtilis
BBa_K1216009BBa_K1216009 Version 1 (Component)Variant of the wild-type pLuxR promoter with lower sensitivity
BBa_K1484011BBa_K1484011 Version 1 (Component)This part is actually benzalacetone reductase. Sorry for the mixup.
BBa_K1051381BBa_K1051381 Version 1 (Component)k1051306(met28 promoter (M phase to G1 phase))+K1051003+ K1051006
BBa_K540003BBa_K540003 Version 1 (Component)rcn-OmpR234, device that triggers adherence in response to cobalt.