BBa_J133650
BBa_J133650 Version 1 (Component)
Chimerabrick Replacement Cassette

BBa_I13452
BBa_I13452 Version 1 (Component)
Adds another BioBrick site

BBa_J133700
BBa_J133700 Version 1 (Component)
primers tail extension EX for BioBricking

BBa_J133701
BBa_J133701 Version 1 (Component)
primers tail extension SP for BioBricking

SgsE | CFP
BBa_K525306 Version 1 (Component)
Fusion Protein of S-Layer SgsE and mCerulean

SbpA | CFP
BBa_K525406 Version 1 (Component)
Fusion Protein of S-Layer SbpA and mCerulean

BBa_K590010
BBa_K590010 Version 1 (Component)
pGA1A3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590011
BBa_K590011 Version 1 (Component)
pGA1C3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590012
BBa_K590012 Version 1 (Component)
pGA4C5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590013
BBa_K590013 Version 1 (Component)
pGA4A5, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

BBa_K590014
BBa_K590014 Version 1 (Component)
pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert

PetroBrick
BBa_K590025 Version 1 (Component)
The PetroBrick: Strong Constitutive Expression of ADC and AAR in pSB1C3

K592200
BBa_K592200 Version 1 (Component)
Low to medium copy BioBrick standard vector

pTG262-BB
BBa_I742123 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector

BBa_K541915
BBa_K541915 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_K541925
BBa_K541925 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and LipA signal peptide

Biosurfact
BBa_K653000 Version 1 (Component)
Re-designing "The Biosurfactator" The first Central American BioBrick

BBa_K812000
BBa_K812000 Version 1 (Component)
Biobricked pCS2+ plasmid for frogs and chicken

BBa_K812001
BBa_K812001 Version 1 (Component)
Biobricked pSC2+ plasmid with pElastase promoter for frog use

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K156001
BBa_K156001 Version 1 (Component)
BioBrick Placeholder 1 (Prefix - ApoI (NotI) AvrII - Suffix)

BBa_K156002
BBa_K156002 Version 1 (Component)
BioBrick placeholder 2 (Prefix - ApoI (NotI) NheI - Suffix)

BBa_K156003
BBa_K156003 Version 1 (Component)
BioBrick placeholder 3 (Prefix - ApoI (NotI) NsiI - Suffix)

BBa_K156004
BBa_K156004 Version 1 (Component)
BioBrick placeholder 4 (Prefix - ApoI (NotI) SbfI - Suffix)

BBa_K156005
BBa_K156005 Version 1 (Component)
BioBrick placeholder 5 (Prefix - MfeI (NotI) AvrII - Suffix)

BBa_K156006
BBa_K156006 Version 1 (Component)
BioBrick placeholder 6 (Prefix - MfeI (NotI) NheI - Suffix)

BBa_K156007
BBa_K156007 Version 1 (Component)
BioBrick placeholder 7 (Prefix - MfeI (NotI) Nsil - Suffix)

BBa_K156008
BBa_K156008 Version 1 (Component)
BioBrick placeholder 8 (Prefix - MfeI (NotI) SbfI - Suffix)

VR2
BBa_J18907 Version 1 (Component)
VR2: Reverse Primer to pSB1 Biobrick Vectors (optimized VR)

pSB1C4
pSB1C4 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_K177015
BBa_K177015 Version 1 (Component)
temp brick

BBa_K249007
BBa_K249007 Version 1 (Component)
CFP with C-terminal Arganine Fusion Tag

BBa_K249009
BBa_K249009 Version 1 (Component)
CFP with N-terminal Arganine Fusion Tag

BBa_K223059
BBa_K223059 Version 1 (Component)
mutTrp (5MT) Inverter with GFP Biobrick part

BBa_K189105
BBa_K189105 Version 1 (Component)
pET-28a+Targeted BioBrick

BBa_K299107
BBa_K299107 Version 1 (Component)
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)

BBa_K299801
BBa_K299801 Version 1 (Component)
standard biobrick scar (if next sequence does NOT start with ATG)

BBa_K299802
BBa_K299802 Version 1 (Component)
Aactag - like a biobrick scar but first T changed to A

BBa_K299804
BBa_K299804 Version 1 (Component)
Cactag - like a biobrick scar but first T changed to C.

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

BBa_K299106
BBa_K299106 Version 1 (Component)
xbaI G (xbaI site and additional G to behave like biobrick prefix after xba digest)

BBa_K299201
BBa_K299201 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between**

BBa_K299301
BBa_K299301 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between**

BBa_K299300
BBa_K299300 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61100[RBS] No b-brick scars between

BBa_K299307
BBa_K299307 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61107[RBS] No b-brick scars between

BBa_K299317
BBa_K299317 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61117[RBS] No b-brick scars between

BBa_K299327
BBa_K299327 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61127[RBS] No b-brick scars between

BBa_K299803
BBa_K299803 Version 1 (Component)
Gactag - like a biobrick scar but first T changed to G

BBa_J95030
BBa_J95030 Version 1 (Component)
BioBrick terminator measurement standard for Rhodobacter sphaeroides