BBa_K077008
BBa_K077008 Version 1 (Component)
Aiia under control of the pcI promotor

BBa_K1315003
BBa_K1315003 Version 1 (Component)
The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>

BBa_J85900
BBa_J85900 Version 1 (Component)
TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4

BBa_K1947020
BBa_K1947020 Version 1 (Component)
This polypeptide will be fused with the recombinant protein which we want to purify.

BBa_M34014
BBa_M34014 Version 1 (Component)
Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana

BBa_K294125
BBa_K294125 Version 1 (Component)
A coding sequence for one of the heat shock protein:hchA

BBa_K777120
BBa_K777120 Version 1 (Component)
motB under the control of constitutive promoter J23114

BBa_K777119
BBa_K777119 Version 1 (Component)
motB under the control of constitutive promoter J23112

BBa_K777118
BBa_K777118 Version 1 (Component)
motB under the control of constitutive promoter J23100

tctB_197aa
BBa_K808005 Version 1 (Component)
tctB_197: small subunit B2 of the tripartite tricarboxylate transporter family

BBa_K422001
BBa_K422001 Version 1 (Component)
Archeal light receptor fused to bacterial chemotaxis transducer

BBa_K1315006
BBa_K1315006 Version 1 (Component)
The <i>manA</i> promoter from <i>Xanthomonas campestris</i>

BBa_K1051115
BBa_K1051115 Version 1 (Component)
ZRC1;Vacuolar Membrane Targeting Protein

BBa_K777124
BBa_K777124 Version 1 (Component)
yhjH under the control of constitutive promoter J23114

BBa_K777123
BBa_K777123 Version 1 (Component)
yhjH under the control of constitutive promoter J23112

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_J64903
BBa_J64903 Version 1 (Component)
Light induced production of RNAi for glnG

BBa_K777111
BBa_K777111 Version 1 (Component)
fliC under the control of constitutive promoter J23112

BBa_K777112
BBa_K777112 Version 1 (Component)
fliC under the control of constitutive promoter J23114

BBa_K1051288
BBa_K1051288 Version 1 (Component)
The measurement pathway of ubiquitin fusion protein.

BBa_K592029
BBa_K592029 Version 1 (Component)
PcpcG2-B0034-BFP green light sensor output

BBa_K2057006
BBa_K2057006 Version 1 (Component)
Expression of gfp under the regulation of promoter BtI-BtII

C6
BBa_K1366105 Version 1 (Component)
Production of survivin siRNA under the hTERT promoter (C6)

BBa_K1172906
BBa_K1172906 Version 1 (Component)
Part 2 of the Biosafety-System araCtive (pBAD Barnase)

BBa_K1555004
BBa_K1555004 Version 1 (Component)
the expression of T7 polymerase controlled by copa promoter

BBa_K2018022
BBa_K2018022 Version 1 (Component)
The gene encoding phasin

BBa_K284022
BBa_K284022 Version 1 (Component)
T4 endolysin under the control of T7 promotor

Propane1
BBa_K1655000 Version 1 (Component)
Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.

OmpA_L
BBa_K257003 Version 1 (Component)
Outer membrane protein A (partial) fused to linker

BBa_I714001
BBa_I714001 Version 1 (Component)
trwC of R388 ( relaxase or nickase of the R388 conjugative plasmid )

BBa_K310007
BBa_K310007 Version 1 (Component)
ArsR repressor of the Ars Operon in E. coli K-12 MG1655

BBa_K284028
BBa_K284028 Version 1 (Component)
LuxS under the control of Py promoter

G
BBa_G0011 Version 1 (Component)
The nucleotide G

A
BBa_G0009 Version 1 (Component)
The nucleotide A

C
BBa_G0010 Version 1 (Component)
The nucleotide C

BBa_K1551000
BBa_K1551000 Version 1 (Component)
To generate delta-4 fatty acid Desaturase so as to synthesize the DHA.

BBa_K1804001
BBa_K1804001 Version 1 (Component)
GFP under the control of constitutive promoter lacI

BBa_K1122001
BBa_K1122001 Version 1 (Component)
GFP (BBa_E0040) under the control of lac promoter

BBa_K1122003
BBa_K1122003 Version 1 (Component)
mTagBFP (BBa_K592100) under the control of lac promoter

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K364304
BBa_K364304 Version 1 (Component)
TRE-CMV

BBa_K777115
BBa_K777115 Version 1 (Component)
motA under the control of constitutive promoter J23112

BBa_K777114
BBa_K777114 Version 1 (Component)
motA under the control of constitutive promoter J23100

BBa_K777116
BBa_K777116 Version 1 (Component)
motA under the control of constitutive promoter J23114

BBa_K1283000
BBa_K1283000 Version 1 (Component)
The protective melittin generator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.