BBa_M45091
BBa_M45091 Version 1 (Component)
AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa

BBa_M31539
BBa_M31539 Version 1 (Component)
These are the genes in M13K07 responsible for forming the 'tip' of the phage.

BBa_J3104
BBa_J3104 Version 1 (Component)
pBad-RBS-HixC-TetF-HixC-TT-RE

BBa_J3103
BBa_J3103 Version 1 (Component)
pBad-HixC-RBS-TetF-HixC-TT-RE

BBa_S03565
BBa_S03565 Version 1 (Component)
hixC-pBADrev-hixC-RBS-TetF-TT-RE

BBa_S03564
BBa_S03564 Version 1 (Component)
hixC-pBAD-hixC-RBS-TetF-TT-RE

BBa_K1058008
BBa_K1058008 Version 1 (Component)
Cr(VI) sensor with egfp(PchrB+chrB+egfp)

BBa_K1778001
BBa_K1778001 Version 1 (Component)
TRE is closely related to promoter regulation,can correctly specific binding to regulatory protein r

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K1961000
BBa_K1961000 Version 1 (Component)
CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli

BBa_S03534
BBa_S03534 Version 1 (Component)
TetB-RBS_rev-HixC-TT-RE

BBa_K325239
BBa_K325239 Version 1 (Component)
Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_S03635
BBa_S03635 Version 1 (Component)
HixC-pBad-HixC-RBS-TetF : HixC-TT-RE

BBa_K325109
BBa_K325109 Version 1 (Component)
EPIC Firefly Luciferase and LRE (under pBAD)<BR><i>P. Pyralis<BR>(E. coli optimised)</i>

BBa_K861160
BBa_K861160 Version 1 (Component)
CRP, cAMP receptor protein

BBa_I741022
BBa_I741022 Version 1 (Component)
CRP gene ORF

BBa_I741111
BBa_I741111 Version 1 (Component)
Lambda Antirepressor Cro

BBa_J3106
BBa_J3106 Version 1 (Component)
pBad-HixC-TetB-RBS_rev-HixC-TT-RE

BBa_I715065
BBa_I715065 Version 1 (Component)
Burnt Pancake Tester with RE and backwards Tet resistance

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_J44008
BBa_J44008 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44006
BBa_J44006 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

BBa_J44007
BBa_J44007 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44005
BBa_J44005 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

BBa_K1434002
BBa_K1434002 Version 1 (Component)
cAMP receptor protein (CRP) mutant

BBa_K364331
BBa_K364331 Version 1 (Component)
TRE-PolyA in pBSB1A3

BBa_S03596
BBa_S03596 Version 1 (Component)
AraCPC_rev-TT : pBad-HixC-RBS-TetF-HixC-TT-RE

BBa_I715069
BBa_I715069 Version 1 (Component)
Test part to see if RE and pLac +hixc can be expressed with GFP

BBa_K1195004
BBa_K1195004 Version 1 (Component)
Cro from Lamda phage with shine delgarno added.

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K1523007
BBa_K1523007 Version 1 (Component)
A Device to reduce Cr(VI)

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.