BBa_K1916019
BBa_K1916019 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916009
BBa_K1916009 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916001
BBa_K1916001 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916018
BBa_K1916018 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

BBa_J92000
BBa_J92000 Version 1 (Component)
Lead Uptake Transporter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J70012
BBa_J70012 Version 1 (Component)
A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

BBa_J70593
BBa_J70593 Version 1 (Component)
RFC12 ATG Head Domain

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K1758332
BBa_K1758332 Version 1 (Component)
Lead responsive promoter with UTR-sfGFP

BBa_K1111013
BBa_K1111013 Version 1 (Component)
Ice Nucleation Protein fused to Streptavidin Dead

BBa_J24808
BBa_J24808 Version 1 (Component)
prbT (lead resistant protein)

BBa_K1051704
BBa_K1051704 Version 1 (Component)
Mer2 Intron

BBa_K1371034
BBa_K1371034 Version 1 (Component)
KS-AT2+DH-ER+KR1+ACP

BBa_K2017000
BBa_K2017000 Version 1 (Component)
C-split Cas9 + DnaE C-intein

BBa_K2017001
BBa_K2017001 Version 1 (Component)
N-split Cas9 + DnaE N-intein

BBa_K824012
BBa_K824012 Version 1 (Component)
Lead Promoter with GFP Reporter (Lead Detector)

BBa_K1758335
BBa_K1758335 Version 1 (Component)
UTR-sfGFP controlled by T7-promoter and lead responsive promoter

VirA/G
BBa_K389017 Version 1 (Component)
VirA/G signaling system without read-out

BBa_J70609
BBa_J70609 Version 1 (Component)
RFC12 7x His Tag Head Domain

BBa_K1362460
BBa_K1362460 Version 1 (Component)
RGK (extein)

BBa_J24801
BBa_J24801 Version 1 (Component)
Lead Detector Gene

BBa_K1362051
BBa_K1362051 Version 1 (Component)
Intein protease with arabinose inducible regulatory promoter/ repressor araC-Pbad-T7RBS-His-SspDnaBC

BBa_K1638036
BBa_K1638036 Version 1 (Component)
Sce VMA Intein with promoter

BBa_K431005
BBa_K431005 Version 1 (Component)
influenza HA globular head

BBa_K1362399
BBa_K1362399 Version 1 (Component)
mutated (c-terminal Ser ->Ala) NpuDnaE C-Intein cloning piece

BBa_K342002
BBa_K342002 Version 1 (Component)
Phasin-Phasin Intein (for silver standard fusion)

BBa_I721003
BBa_I721003 Version 1 (Component)
Lead Binding Protein + Promoter (version b)

BBa_K1961006
BBa_K1961006 Version 1 (Component)
Lead ion detector

FLAG
BBa_T2004 Version 1 (Component)
FLAG octapeptide head domain (DYKDDDDK)

c-Myc
BBa_T2007 Version 1 (Component)
c-Myc head domain (EQKLISEEDL)