BBa_M11406
BBa_M11406 Version 1 (Component)
Type 1 promoter of psaAB operon in Synechocystis sp. PCC 6803

BBa_M11407
BBa_M11407 Version 1 (Component)
Type 1 promoter of hspA gene in Synechocystis sp. PCC 6803

BBa_M41009
BBa_M41009 Version 1 (Component)
Genome sequence of long direct repeat sequence - D which encodes LdrD.

BBa_M11405
BBa_M11405 Version 1 (Component)
Type 1 promoter of petBD operon in Synechocystis sp. PCC 6803

BBa_M33002
BBa_M33002 Version 1 (Component)
CDS for Fnr-LH28 protein, activates narGp in presence of nitrate.

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

RBS+eGFP+T
BBa_K193205 Version 1 (Component)
TmTHP with EGFP for fusion of Anti-Freeze Protein and RBS

BBa_M11408
BBa_M11408 Version 1 (Component)
Type 1 promoter of sigA gene in Synechocystis sp. PCC 6803

BBa_M11404
BBa_M11404 Version 1 (Component)
Type 1 promoter of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K1861040
BBa_K1861040 Version 1 (Component)
Fusion protein of SpyCatcher and RPD3 in an expression cassette

BBa_J64713
BBa_J64713 Version 1 (Component)
LasR/LasI Inducible (RHLR/RHLI repressible) positive feedback production of 3OC12 HSL

BBa_M45138
BBa_M45138 Version 1 (Component)
Type II Secretion of Chromate and Uranium Reductase with TorA

BBa_K1165001
BBa_K1165001 Version 1 (Component)
GFP Fluorescent Protein under the Control of the pOmpC Promoter

BBa_K237002
BBa_K237002 Version 1 (Component)
Constitutive RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

BBa_K2082238
BBa_K2082238 Version 1 (Component)
Beta-lactamase under the control of a modified lacZ promoter

BBa_M39023
BBa_M39023 Version 1 (Component)
AND gate structure for regulation of E.coli in CF mucus

BBa_K1113002
BBa_K1113002 Version 1 (Component)
Targeting sequence for the delivery of GFP to the Carboxysome

BBa_K1707005
BBa_K1707005 Version 1 (Component)
TetR repressor controlling the expression of CFP tagged with LVA

BBa_K1960050
BBa_K1960050 Version 1 (Component)
Low-level expression of LuxR and the lux pR promoter

BBa_K1960051
BBa_K1960051 Version 1 (Component)
High-level expression of LuxR and the lux pR promoter

BBa_K237004
BBa_K237004 Version 1 (Component)
Constitutive sRIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

BBa_K152005
BBa_K152005 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y and GFP

BBa_J64714
BBa_J64714 Version 1 (Component)
RHLR/RHLI Inducible (LasR/LasI repressible) positive feedback production of C4 HSL

BBa_K079031
BBa_K079031 Version 1 (Component)
GFP reporter protein under the control of the BBa_J23118 promoter

SPtim21
BBa_K809203 Version 1 (Component)
signal peptide of TIM21

BBa_I13213
BBa_I13213 Version 1 (Component)
BioBricked version of the natural Lux system with order reversed

Orai1 V102
BBa_K2028000 Version 1 (Component)
This part is the cDNA of a mutated calcium ion channel protein.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1407003
BBa_K1407003 Version 1 (Component)
constitutive production of LasR +LasR/PAI1 Inducible promoter+RBS(B0030)+RFP

BBa_K1407006
BBa_K1407006 Version 1 (Component)
constitutive production of LasR + LasR/PAI1 Inducible promoter +RBS(B0034)+GFP

BBa_K2047104
BBa_K2047104 Version 1 (Component)
Fluorescent reporter system with stem-loop of -34.4kcal/mol, GFP_stem-loop

BBa_K2047101
BBa_K2047101 Version 1 (Component)
Fluorescent reporter system with stem-loop of -30.1kcal/mol, GFP_stem-loop

BBa_K2151010
BBa_K2151010 Version 1 (Component)
p25-B0034-K592009: Blue chromoprotein under control of medium constitutive promoter

BBa_K2151009
BBa_K2151009 Version 1 (Component)
pHLBA-B0034-K592009: Blue chromoprotein under control of strong constitutive promoter

BBa_K2047108
BBa_K2047108 Version 1 (Component)
Fluorescent reporter system with stem-loop of -38.8kcal/mol, GFP_Stem-loop

BBa_K2151011
BBa_K2151011 Version 1 (Component)
p32-B0034-K592009: Blue chromoprotein under control of strong constitutive promoter

BBa_K2047102
BBa_K2047102 Version 1 (Component)
Fluorescent reporter system with stem loop of -14.9kcal/mol,GFP_Stem-loop

BBa_K2047105
BBa_K2047105 Version 1 (Component)
Fluorescent reporter system with stem loop of -51.4kcal/mol, GFP_Stem-loop

BBa_I20257
BBa_I20257 Version 1 (Component)
Autoregulated synthesis of T7 RNAP with lacI negative feedback (orthogonal translation)

BBa_K1111007
BBa_K1111007 Version 1 (Component)
MMP2 under the control of the pBAD/araC arabinose inducible promoter

BBa_K608000
BBa_K608000 Version 1 (Component)
Second of two required phycocyanobilin biosynthetic genes combined with a terminator

BBa_K2047103
BBa_K2047103 Version 1 (Component)
Fluorescent reporter system with stem-loop of -38.8kcal/mol, GFP_Stem-loop

FBS-AceB+L
BBa_K1163999 Version 1 (Component)
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA

BBa_K195402
BBa_K195402 Version 1 (Component)
This part is composed of RBS,Lpp-OmpA-linker and F10scFv

BBa_K2016005
BBa_K2016005 Version 1 (Component)
Superfolder GFP under control of strong constitutive promoter and RyhB siRNA domain

BBa_K1497005
BBa_K1497005 Version 1 (Component)
B0034-CHS-B0034-CHI - Composite part of RBS-CHS and RBS-CHI

BBa_J45013
BBa_J45013 Version 1 (Component)
PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid

BBa_J45011
BBa_J45011 Version 1 (Component)
PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid

BBa_I20249
BBa_I20249 Version 1 (Component)
Device used to characterize Ribosome Binding Sites through measurement of GFP expression

BBa_K1963010
BBa_K1963010 Version 1 (Component)
An sRNA system for down-regulation of E. coli fliC encoding flagellin