BBa_K1961000
BBa_K1961000 Version 1 (Component)
CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli

mCherry
BBa_K105010 Version 1 (Component)
mCherry, Yeast optimized for fusion proteins

BBa_S03534
BBa_S03534 Version 1 (Component)
TetB-RBS_rev-HixC-TT-RE

BBa_K105008
BBa_K105008 Version 1 (Component)
EYFP, yeast optimized for fusion proteins

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

BBa_S03635
BBa_S03635 Version 1 (Component)
HixC-pBad-HixC-RBS-TetF : HixC-TT-RE

BBa_I716212
BBa_I716212 Version 1 (Component)
Cre (GTG start)

BBa_K1820005
BBa_K1820005 Version 1 (Component)
mCherry(Lr)

BBa_J3106
BBa_J3106 Version 1 (Component)
pBad-HixC-TetB-RBS_rev-HixC-TT-RE

BBa_I715065
BBa_I715065 Version 1 (Component)
Burnt Pancake Tester with RE and backwards Tet resistance

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K2152003
BBa_K2152003 Version 1 (Component)
Bacteriophage Phi X 174 lysis gene E(wild type)

BBa_K1695042
BBa_K1695042 Version 1 (Component)
Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_J44008
BBa_J44008 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44006
BBa_J44006 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

BBa_J44007
BBa_J44007 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44005
BBa_J44005 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

BBa_K2036028
BBa_K2036028 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase

BBa_K364331
BBa_K364331 Version 1 (Component)
TRE-PolyA in pBSB1A3

BBa_K2036027
BBa_K2036027 Version 1 (Component)
pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag

BBa_K886002
BBa_K886002 Version 1 (Component)
Recombination Device composed by Cre-lox71

BBa_K2036024
BBa_K2036024 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase

BBa_S03596
BBa_S03596 Version 1 (Component)
AraCPC_rev-TT : pBad-HixC-RBS-TetF-HixC-TT-RE

BBa_K242300
BBa_K242300 Version 1 (Component)
multi-Ler binding site +promoter 1

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_I715069
BBa_I715069 Version 1 (Component)
Test part to see if RE and pLac +hixc can be expressed with GFP

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.