BBa_J40001
BBa_J40001 Version 1 (Component)
LacI (LVA) + ECFP (LVA) under luxpR promoter (LuxR/HSL regulated)

BBa_K228813
BBa_K228813 Version 1 (Component)
RBS(B0034)+lacI(C0012)+terminator(B0015)

BBa_K318509
BBa_K318509 Version 1 (Component)
lacI pL + RBS + cI LVA + TT

BBa_K1675023
BBa_K1675023 Version 1 (Component)
P-atp2 mutant 706-B0034-lacz alpha

BBa_K1675022
BBa_K1675022 Version 1 (Component)
P-atp2 mutant 317-B0034-lacz alpha

BBa_K1675025
BBa_K1675025 Version 1 (Component)
P-atp2 mutant 399-B0034-lacz alpha

BBa_K1675024
BBa_K1675024 Version 1 (Component)
P-atp2 mutant 389-B0034-lacz alpha

BBa_K1675020
BBa_K1675020 Version 1 (Component)
P-atp2 mutant 226-B0034-lacz alpha

RBS-LacI-T
BBa_K079002 Version 1 (Component)
LacI protein with terminator and RBS

BBa_K934002
BBa_K934002 Version 1 (Component)
LuxR/3OC6HSL complex dependent LacI generator

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

BBa_I714074
BBa_I714074 Version 1 (Component)
[R0010][J23066] ([pLac][Key3][Double Terminator])

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_K1676100
BBa_K1676100 Version 1 (Component)
GFP Reporter with pLac as promoter

BBa_S03609
BBa_S03609 Version 1 (Component)
AraCPCrev-TT : pLac-RBS-HinLVA-TT

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_K188122
BBa_K188122 Version 1 (Component)
RBS+lacI repressor from E. coli (+LVA)

BBa_K329005
BBa_K329005 Version 1 (Component)
Strong RBS (B0034) - LacZ-alpha fragment (I732006)

BBa_K1141000
BBa_K1141000 Version 1 (Component)
Plac-RBS-mCherry-double terminator (IPTG-inducible)

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_K199170
BBa_K199170 Version 1 (Component)
T7 Promoter RBS RFP pLac CCACC (tRNA)

BBa_K199169
BBa_K199169 Version 1 (Component)
T7 Promoter RBS RFP pLac CUAGC (tRNA)

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_I732091
BBa_I732091 Version 1 (Component)
Double Repoters (LacZ-alpha and GFP-AAV)

BBa_K564016
BBa_K564016 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564017
BBa_K564017 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K1472613
BBa_K1472613 Version 1 (Component)
∆9, ∆12, ∆15 desaturases with LacI promoter

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_K1952012
BBa_K1952012 Version 1 (Component)
Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter

BBa_J47053
BBa_J47053 Version 1 (Component)
Constitutive device (medium transcription) for lacI repressor, strong RBS

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

pTetR-RFP-
BBa_I763040 Version 1 (Component)
RFP regulated by TetR and GFP regulated by pLac

BBa_K132016
BBa_K132016 Version 1 (Component)
luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator

BBa_K137021
BBa_K137021 Version 1 (Component)
GFP with (AC)20 repeat after start codon

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

FBS-AceB+L
BBa_K1163999 Version 1 (Component)
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.