pIGEM010BBa_K1537010 Version 1 (Component)piGEM002+TCP02-HPS-PHI+Tp-AtFDH+FALDH
BBa_K1905004BBa_K1905004 Version 1 (Component)Riboswitch to detect L1 mRNA from HPV and express BFP
BBa_K1905005BBa_K1905005 Version 1 (Component)Riboswitch to detect E6 mRNA from HPV and express YFP
ErbB1 (SP)BBa_K157001 Version 1 (Component)EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore
BBa_J176058BBa_J176058 Version 1 (Component)luc miR sensor 6 (R/HPK-T/CMV)
BBa_J176056BBa_J176056 Version 1 (Component)luc miR sensor 4 (R/HPK-T/Ubc)
BBa_J176054BBa_J176054 Version 1 (Component)luc miR sensor 2 (R/Ubc-T/HPK)
BBa_M12069BBa_M12069 Version 1 (Component)hin invertase under constitutive promoter pspv from Salmonella
BBa_M12070BBa_M12070 Version 1 (Component)hin invertase under constitutive promoter pspv from Salmonella
BBa_M12071BBa_M12071 Version 1 (Component)hin invertase under constitutive promoter pspv from Salmonella
BBa_K318028BBa_K318028 Version 1 (Component)hixC + pCons + lox66 + Hin enhancer + SapI + lox71 + hixC
BBa_K906003BBa_K906003 Version 1 (Component)Histidine Patch Thioredoxins (HP-Thioredoxin)
BBa_E70101BBa_E70101 Version 1 (Component)GFP expression system that uses dedicated translation
BBa_K1091001BBa_K1091001 Version 1 (Component)green fluorescent protein with a fast translation speed
BBa_K1905003BBa_K1905003 Version 1 (Component)Riboswitch to detect E5 mRNA from HPV-16 and express RFP
BBa_K524010BBa_K524010 Version 1 (Component)E. coli Translation Initiation Factor 1 (IF1) Operon (infA)
BBa_K1162014BBa_K1162014 Version 1 (Component)protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon
BBa_K1758102BBa_K1758102 Version 1 (Component)Translation enhancing 5-UTR + sfGFP under control of T7 promoter
PN+E0240BBa_K100004 Version 1 (Component)Natural Xylose Regulated Bi-Directional Operator + GFP
"Lock"BBa_K318030 Version 1 (Component)lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC
BBa_J44007BBa_J44007 Version 1 (Component)pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF
BBa_J44005BBa_J44005 Version 1 (Component)pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
BBa_I20257BBa_I20257 Version 1 (Component)Autoregulated synthesis of T7 RNAP with lacI negative feedback (orthogonal translation)
BBa_K318521BBa_K318521 Version 1 (Component)Encryption Lysis - pLock Lysis p(pH) Hin pBile Cre pCons ramA
LacImBBa_K092800 Version 1 (Component)Coding sequence for LacI modified with a different rate of translation than LacI, RBS
BBa_K1698001BBa_K1698001 Version 1 (Component)BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp
BBa_K1613012BBa_K1613012 Version 1 (Component)QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.
BBa_I715048BBa_I715048 Version 1 (Component)Hin + pT7 -> RFP1 + GFP1 medium initial orientation
BBa_I715050BBa_I715050 Version 1 (Component)Hin + pT7 -> RFP1 + GFP1 medium initial orientation
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.