BBa_Y00029
BBa_Y00029 Version 1 (Component)
S. pombe homolog of S.cerevisiae SGF29, recoded for expression in S.c.

BBa_K1509000
BBa_K1509000 Version 1 (Component)
Coding for the trans-acting regulator SmtB

S-protein
BBa_M0029 Version 1 (Component)
S-protein affinity tag

ComE
BBa_K1379051 Version 1 (Component)
<i>S. pneumoniae</i> ComE CDS

BBa_J24688
BBa_J24688 Version 1 (Component)
9. S RBS - TetR

BBa_K1460002
BBa_K1460002 Version 1 (Component)
RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter

IBMc318
IBMc318 Version 1 (Component)
pSB1A3-BbsI-G-ER-LBD-S-SapI

BBa_J24716
BBa_J24716 Version 1 (Component)
36. pLacI=W RBS=AraC=S RBS=TetR=S RBS=mCherry(+LVA)=terminator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1871002
BBa_K1871002 Version 1 (Component)
Alpha amylase with mating factor alpha secretion

BBa_K2018003
BBa_K2018003 Version 1 (Component)
Thuricin S

IGPD
BBa_K1154002 Version 1 (Component)
Saccharomyces cerevisiae imidazoleglycerol-phosphate dehydratase (IGPD)

BBa_K2151002
BBa_K2151002 Version 1 (Component)
p32, strong constitutive <i>S. thermophilus</i> promoter

BBa_K2151001
BBa_K2151001 Version 1 (Component)
p25, moderate constitutive <i>S. thermophilus</i> promoter

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

S-tag
BBa_T2017 Version 1 (Component)
S-tag special internal domain (KETAAAKFERQHMDS)

S-tag
BBa_T2016 Version 1 (Component)
S-tag head domain (KETAAAKFERQHMDS)

BBa_J329002
BBa_J329002 Version 1 (Component)
Intermediate part for making a QS system

S-tag
BBa_T2015 Version 1 (Component)
S-tag tail domain (KETAAAKFERQHMDS)

BBa_K1051300
BBa_K1051300 Version 1 (Component)
cln2 promoter (G1 phase to S phase)

BBa_K1900005
BBa_K1900005 Version 1 (Component)
S. marcescens tolC

BBa_M11207
BBa_M11207 Version 1 (Component)
Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae

BBa_M11208
BBa_M11208 Version 1 (Component)
Device containing HXT1/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K1051306
BBa_K1051306 Version 1 (Component)
met28 promoter (S phase)

BBa_K1097000
BBa_K1097000 Version 1 (Component)
adh2&#65288;Saccharomyces cerevisiae&#65289;

BBa_K2018008
BBa_K2018008 Version 1 (Component)
Laterosporulin-Thuricin S

BBa_K1014003
BBa_K1014003 Version 1 (Component)
HrpR Protein Generator(S)

BBa_K1014011
BBa_K1014011 Version 1 (Component)
RFP Protein Generator(S)

BBa_K917014
BBa_K917014 Version 1 (Component)
pLac promoter plus S. oneidensis tetraheme cytochrome c cymA

S + P25 +
BBa_K320014 Version 1 (Component)
S + P25 + P28 + Ori + Tet

TEF2 pr
BBa_K1154005 Version 1 (Component)
Saccharomyces cerevisiae constitutive TEF2 promoter variant

BBa_J176026
BBa_J176026 Version 1 (Component)
5xLuc s-target

BBa_K358014
BBa_K358014 Version 1 (Component)
S&#916;TMD1RRz

BBa_K1351000
BBa_K1351000 Version 1 (Component)
beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC

BBa_K2158001
BBa_K2158001 Version 1 (Component)
Allantoinase from <i>Saccharomyces cerevisiae

BBa_J24682
BBa_J24682 Version 1 (Component)
3. S RBS - AraC

BBa_K2151000
BBa_K2151000 Version 1 (Component)
pHLBA, strong constitutive <i>S. thermophilus</i> promoter

BBa_J24706
BBa_J24706 Version 1 (Component)
26. M RBS-TetR-S RBS-mCherry(+LVA)-terminator

BBa_M45181
BBa_M45181 Version 1 (Component)
S-ribosylhomocysteine lyase

BBa_K896902
BBa_K896902 Version 1 (Component)
Against aging!!

BBa_J24711
BBa_J24711 Version 1 (Component)
31. W RBS - LacI - S RBS - EYFP(+LVA) - terminator

BBa_J24712
BBa_J24712 Version 1 (Component)
32. M RBS - LacI - S RBS - EYFP(+LVA) - terminator

BBa_J23006
BBa_J23006 Version 1 (Component)
Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI

BBa_K1351001
BBa_K1351001 Version 1 (Component)
Contactin-4-derived peptide binding to NADH Oxidase of S. pneumoniae

BBa_J24708
BBa_J24708 Version 1 (Component)
28. W RBS - TetR - S RBS - ECFP(+LVA) - terminator

BBa_J24709
BBa_J24709 Version 1 (Component)
29. M RBS - TetR - S RBS - ECFP (+LVA) - terminator

BBa_K1526001
BBa_K1526001 Version 1 (Component)
Gsh1*: Mutated Glutamate-Cysteine Ligase(Gsh1) from Saccharomyces cerevisiae

SAMmt
BBa_K1312005 Version 1 (Component)
S-adenosyl methionine-dependent methyltransferase relate to Met-DMSP byosynthesis pathway(SAMmt)

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast