BBa_K1916009BBa_K1916009 Version 1 (Component)Osc7112_5903g3 + intein-CBD tag
BBa_K1916001BBa_K1916001 Version 1 (Component)NpF2164g5 + intein-CBD tag
BBa_K1916018BBa_K1916018 Version 1 (Component)CBCR8 + intein-CBD tag
BBa_K1916013BBa_K1916013 Version 1 (Component)CBCR3 + intein-CBD tag
BBa_K1916007BBa_K1916007 Version 1 (Component)Ga0039498_104087g2 + intein-CBD tag
BBa_K1916014BBa_K1916014 Version 1 (Component)CBCR4 + intein-CBD tag
BBa_K1916000BBa_K1916000 Version 1 (Component)NpF2164g5 + intein-CBD tag
BBa_K1916003BBa_K1916003 Version 1 (Component)Cha6605_4344g2 + intein-CBD tag
BBa_K1916006BBa_K1916006 Version 1 (Component)Ga0039498_104087g2 + intein-CBD tag
BBa_K1435000BBa_K1435000 Version 1 (Component)4-HT inducible intein
BBa_K1916002BBa_K1916002 Version 1 (Component)Cyan7427_1390g3 + intein-CBD tag
BBa_K1916021BBa_K1916021 Version 1 (Component)CBCR9 + intein-CBD tag
BBa_K1916004BBa_K1916004 Version 1 (Component)Ga0100955_11928g3 + intein-CBD tag
BBa_K1916016BBa_K1916016 Version 1 (Component)CBCR6 + intein-CBD tag
BBa_K1916012BBa_K1916012 Version 1 (Component)CBCR3 + intein-CBD tag
BBa_K1073018BBa_K1073018 Version 1 (Component)Autoinducer synthetase LasI ( producing N-3-oxododecanoyl-HSL) with RBS and double terminator
BBa_K2033002BBa_K2033002 Version 1 (Component)isovaleryl-HSL, 3-methyl-N-[(3S)-2-oxooxolan-3-yl]butanamide Sender- BjaI
BBa_K902059BBa_K902059 Version 1 (Component)katG +LAA behind an RBS (B0034)
BBa_K331011BBa_K331011 Version 1 (Component)Catechol 2,3-dioxygenase with a C-terminus arginine tag
BBa_K368009BBa_K368009 Version 1 (Component)tet-on Promoter+TEV recogn.site+N-degron+SF3b155+Human Bak+SV40PA
BBa_J70628BBa_J70628 Version 1 (Component)RFC12 Sce VMA Intein Internal Domain
BBa_K1159111BBa_K1159111 Version 1 (Component)Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-Part
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
M13 -47BBa_K176059 Version 1 (Component)M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ
BBa_J04451BBa_J04451 Version 1 (Component)RFP Coding Device with an LVA tag
BBa_K1159014BBa_K1159014 Version 1 (Component)Membrane-anchored Erythromycin Esterase Type II in RFC[25] N-Part
BBa_K1159008BBa_K1159008 Version 1 (Component)Secretory Catechol-2,3-dioxygenase (IgKappa-SigP_XylE) in RFC[25] N-Part
BBa_K1159018BBa_K1159018 Version 1 (Component)Membrane-anchored Catechol-2,3-dioxygenase (XylE) in RFC[25] N-Part
BBa_K1053120BBa_K1053120 Version 1 (Component)RNA scaffold containing HTLV-1 Rex peptide and λN peptide aptamer
BBa_J07075BBa_J07075 Version 1 (Component)PhoA'' (w/out EcoRI sites)
RGKBBa_K1362434 Version 1 (Component)RGK (extein)
BBa_K1679027BBa_K1679027 Version 1 (Component)VMA, a thermosensitive intein
BBa_K1125000BBa_K1125000 Version 1 (Component)SrUCPa w/ kozack sequence
BBa_K165001BBa_K165001 Version 1 (Component)pGAL1+ w/XhoI sites
BBa_J64850BBa_J64850 Version 1 (Component)SP6 gene w/ RBS
BBa_J07046BBa_J07046 Version 1 (Component)4M5.3 scFv (J07014) with linker at C terminus
BBa_K2170201BBa_K2170201 Version 1 (Component)Single chain avidin with ribosomal binding site (RBS_scAvidin) in RFC[25] N-part
BBa_K1957007BBa_K1957007 Version 1 (Component)HyaB C-terminally tagged subunit of NiFe Hydrogenase
BBa_K1957002BBa_K1957002 Version 1 (Component)HydB (C-terminally tagged) subunit of FeFe Hydrogenase
BBa_K1162106BBa_K1162106 Version 1 (Component)LL-37 antimicrobial peptide generator with C-terminal 10x His-Tag for purification
BBa_K1694053BBa_K1694053 Version 1 (Component)Pcons+B0034+Lpp-OmpA-N+scFv(Anti-VEGF)+B0034+amilCP+B0015
BBa_K110001BBa_K110001 Version 1 (Component)Between-bud 27-W FRS2-C
BBa_K2066536BBa_K2066536 Version 1 (Component)Spinach Aptamer w/ tRNA Scaffold
BBa_K382027BBa_K382027 Version 1 (Component)Arabidopsis optimized Miraculin with StrepII C-terminus tag
BBa_K1362300BBa_K1362300 Version 1 (Component)NpuDnaE N-Intein RFC[105] assembly construct (with His6)
BBa_K1491027BBa_K1491027 Version 1 (Component)T7 RNA Polymerase with Dead ER Intein
BBa_J52021BBa_J52021 Version 1 (Component)dnTraf6-linker-GFP
BBa_K1173502BBa_K1173502 Version 1 (Component)<i>ompC</i> w/GFP + (<i>BglII+BamHI</i>)restriction sites