BBa_K575038
BBa_K575038 Version 1 (Component)
RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0030) + RhlR

BBa_K575039
BBa_K575039 Version 1 (Component)
RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0034) + RhlR

BBa_K575040
BBa_K575040 Version 1 (Component)
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP

BBa_K575041
BBa_K575041 Version 1 (Component)
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP

BBa_K575042
BBa_K575042 Version 1 (Component)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP

BBa_K575043
BBa_K575043 Version 1 (Component)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP

BBa_K575044
BBa_K575044 Version 1 (Component)
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR

BBa_K575045
BBa_K575045 Version 1 (Component)
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR

BBa_K575046
BBa_K575046 Version 1 (Component)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR

BBa_K575047
BBa_K575047 Version 1 (Component)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP, Cons. Promoter + RBS (B0034) + RhlR

BBa_K566005
BBa_K566005 Version 1 (Component)
pCpcG2 promoter (Green light inducible)

pCpcG2 inv
BBa_K566009 Version 1 (Component)
pCpcG2 promoter, inverted sequence (Green light inducible)

BBa_K823015
BBa_K823015 Version 1 (Component)
Pxyl, a xylose inducible promoter + its regulator XylR

BBa_K748006
BBa_K748006 Version 1 (Component)
S.aureus killing device. Truncated lysostaphin coding sequence with IPTG inducible promoter.

BBa_K748007
BBa_K748007 Version 1 (Component)
Biofilm formation device. yddV gene with the IPTG inducible promoter.

BBa_K764020
BBa_K764020 Version 1 (Component)
Inducible pBad/araC promoter + BBa_B0034

BBa_J100097
BBa_J100097 Version 1 (Component)
Anhydrotetracycline inducible promoter with BsaI sticky ends

BBa_K733002
BBa_K733002 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.

BBa_K737004
BBa_K737004 Version 1 (Component)
Inducible GVPA Generator

BBa_K737005
BBa_K737005 Version 1 (Component)
Inducible GVPC Generator

BBa_K737051
BBa_K737051 Version 1 (Component)
IPTG inducible GFP Generator

BBa_K737052
BBa_K737052 Version 1 (Component)
aTc inducible GFP Generaror

BBa_K759001
BBa_K759001 Version 1 (Component)
Aggregation Module inducible by arabinose in E.coli.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K131019
BBa_K131019 Version 1 (Component)
LuxO inducible RFP

BBa_K131026
BBa_K131026 Version 1 (Component)
AHL inducible GFP

BBa_K090501
BBa_K090501 Version 1 (Component)
Gram-Positive IPTG-Inducible Promoter

BBa_K090502
BBa_K090502 Version 1 (Component)
Gram-Positive Xylose-Inducible Promoter

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

BBa_K116102
BBa_K116102 Version 1 (Component)
IPTG inducible membrane protein generator

BBa_K165042
BBa_K165042 Version 1 (Component)
Gli1 binding sites + MET25 inducible yeast promoter

BBa_J69022
BBa_J69022 Version 1 (Component)
Lux Auto-inducible Device 3

BBa_J69150
BBa_J69150 Version 1 (Component)
Las Sender Device - Arabinose Inducible

BBa_J70301
BBa_J70301 Version 1 (Component)
Heat shock inducible promoter for Me. florum

BBa_J69536
BBa_J69536 Version 1 (Component)
Arabinose-inducible tetR Generator

Pfec
BBa_K257000 Version 1 (Component)
fec promoter (Pfec) - inducible through the Fec transduction pathway (ferric citrate)

BBa_K237001
BBa_K237001 Version 1 (Component)
IPTG inducible RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

BBa_K237006
BBa_K237006 Version 1 (Component)
IPTG inducible sRIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_K174001
BBa_K174001 Version 1 (Component)
Arabinose inducible system

BBa_K177046
BBa_K177046 Version 1 (Component)
Tetracycline repressor under AraC regulated promoter

BBa_K173021
BBa_K173021 Version 1 (Component)
pdc and adhB inducible system (HSL)

BBa_K173022
BBa_K173022 Version 1 (Component)
adhB and pdc inducible system (HSL)

BBa_K173023
BBa_K173023 Version 1 (Component)
pdc inducible system (HSL)

BBa_K173024
BBa_K173024 Version 1 (Component)
adhB inducible system (HSL)