BBa_K137085
BBa_K137085 Version 1 (Component)
optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements

BBa_K1362444
BBa_K1362444 Version 1 (Component)
GGG linker with RGK (extein)

BBa_K1758342
BBa_K1758342 Version 1 (Component)
Mercury responsive promoter with UTR-sfGFP

BBa_K861169
BBa_K861169 Version 1 (Component)
Indirect regulatory device, activated at high glucose concentration

BBa_K300083
BBa_K300083 Version 1 (Component)
Phasins (separated by a flexible protein domain linker) with intein downstream

BBa_K861022
BBa_K861022 Version 1 (Component)
FadE with RBS and terminator

BBa_K1903019
BBa_K1903019 Version 1 (Component)
Intein N SSp DnaB S1

BBa_K1592017
BBa_K1592017 Version 1 (Component)
Mcfp3 with XPR2 pre

BBa_J70577
BBa_J70577 Version 1 (Component)
C-terminal protein tag addition test circuit

BBa_K1362108
BBa_K1362108 Version 1 (Component)
RBS + NpuDnaE N&C-intein coexpression RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_K1692006
BBa_K1692006 Version 1 (Component)
UbiX with T7 Promoter

BBa_K1692003
BBa_K1692003 Version 1 (Component)
PAL with T7 promoter

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K1399006
BBa_K1399006 Version 1 (Component)
GFP (mut3b) with LAA-ssrA degradation tag

BBa_K1399008
BBa_K1399008 Version 1 (Component)
GFP (mut3b) with DAS-ssrA degradation tag

BBa_J04606
BBa_J04606 Version 1 (Component)
Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)

BBa_I746100
BBa_I746100 Version 1 (Component)
AIP sensor infrastructure (agrC + agrA from S. aureus with RBSes)

BBa_K523000
BBa_K523000 Version 1 (Component)
Plac + lacZ; with BglII site

BBa_K642000
BBa_K642000 Version 1 (Component)
TetR repressor tagged with yBFP

BBa_K642002
BBa_K642002 Version 1 (Component)
cI repressor tagged with yBFP

BBa_K542000
BBa_K542000 Version 1 (Component)
Lumazine Synthase with Transciptional Terminator (No Promoter)

BBa_K216006
BBa_K216006 Version 1 (Component)
pentaerythritol tetranitrate reductase with native rbs

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_P10000
BBa_P10000 Version 1 (Component)
LexA promoter with 5' UTR

BBa_K1931013
BBa_K1931013 Version 1 (Component)
T7-RBS-Epidermicin NI01-MBP-Terminator

BBa_K1931014
BBa_K1931014 Version 1 (Component)
T7-RBS-Microcin E492-MBP-Terminator

BBa_K953002
BBa_K953002 Version 1 (Component)
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)

BBa_K622004
BBa_K622004 Version 1 (Component)
glnLG: coding both nitrogen regulatory protein NtrB and NtrC

BBa_K1362161
BBa_K1362161 Version 1 (Component)
RBS + Gp41-1 C-intein RFC[105] assembly construct (with His6)

BBa_I742116
BBa_I742116 Version 1 (Component)
crtE with native ribosome binding site

BBa_K549005
BBa_K549005 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_K316027
BBa_K316027 Version 1 (Component)
B.subtilis transformation vector with LacI

BBa_K1051974
BBa_K1051974 Version 1 (Component)
clb6 P + D-box + GFP + MER2 Intron + TOM40 + T

BBa_K1362109
BBa_K1362109 Version 1 (Component)
RBS + NpuDnaE N&C-Intein coexpression RFC[105] nonsplicing assembly construct (with His6)

BBa_K1758312
BBa_K1758312 Version 1 (Component)
Chromium responsive promoter with UTR+RBS and sfGFP

BBa_K2088005
BBa_K2088005 Version 1 (Component)
pbaC with double Terminator

BBa_K199008
BBa_K199008 Version 1 (Component)
CCAUC Suppressor tRNA (10-bp Anticodon and Produces Serine)

BBa_K118018
BBa_K118018 Version 1 (Component)
rbs+glgC16 (glgC with G336D substitution)

BBa_K137049
BBa_K137049 Version 1 (Component)
450 bp inverted tetR promoter

BBa_K137048
BBa_K137048 Version 1 (Component)
350 bp inverted tetR promoter

BBa_K137050
BBa_K137050 Version 1 (Component)
650 bp inverted tetR promoter

BBa_K137051
BBa_K137051 Version 1 (Component)
850 bp inverted tetR promoter

rbs + sam5
BBa_K238007 Version 1 (Component)
Sam5 gene with RBS

BBa_K648101
BBa_K648101 Version 1 (Component)
RecA (mutated from RecA1 at amino acid 160 G-->A

BBa_K342003
BBa_K342003 Version 1 (Component)
OmpR234 protein, with higher effect on Curli promoter

BBa_K812032
BBa_K812032 Version 1 (Component)
mCFP with kozak sequence for eukaryotic expression

BBa_J32009
BBa_J32009 Version 1 (Component)
4HT Dependent Intein

BBa_K541596
BBa_K541596 Version 1 (Component)
Reflectin gene with J04500 promoter

BBa_K1321111
BBa_K1321111 Version 1 (Component)
Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter