BBa_K1159201
BBa_K1159201 Version 1 (Component)
Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]

BBa_K1321341
BBa_K1321341 Version 1 (Component)
sfGFP fused to CBDclos in RFC 25

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_K1159101
BBa_K1159101 Version 1 (Component)
N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

BBa_K1159001
BBa_K1159001 Version 1 (Component)
NanoLuc Luciferase in RFC[25]

RFC105 CN
BBa_K1362418 Version 1 (Component)
N-terminal GG-linker overhang <u>GGTG</u>=Gly+1/3Gly RFC[105] overhang CN

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

TEV clv.
BBa_K1362453 Version 1 (Component)
TEV protease recognition/cleavage site

BBa_K1159310
BBa_K1159310 Version 1 (Component)
34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]

BBa_K1159102
BBa_K1159102 Version 1 (Component)
C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

RFC105 NC
BBa_K1362417 Version 1 (Component)
C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC

BBa_K1321349
BBa_K1321349 Version 1 (Component)
CBDcex fused to sfGFP in RFC 25

BBa_K1321343
BBa_K1321343 Version 1 (Component)
NiBP fused to CBDclos in RFC 25

BBa_K1321344
BBa_K1321344 Version 1 (Component)
NiBP fused to CBDcex in RFC 25

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_K648009
BBa_K648009 Version 1 (Component)
RecA Cleavage Site with Standard 25 Prefix/Suffix

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K1159112
BBa_K1159112 Version 1 (Component)
FRET Reporter for TEV Protease activity (eCFP_TEV-site-linker_SYFP2) in RFC[25]

BBa_K1321346
BBa_K1321346 Version 1 (Component)
CBDclos fused to sfGFP in RFC 25

BBa_K1159316
BBa_K1159316 Version 1 (Component)
SERK-Receptor TMD with C-terminal GFP and N-terminal <i>Strep</i>-tag II TEV-site linker in RFC[25]

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BsaI ->
BBa_K1362424 Version 1 (Component)
BsaI restriction site for RFC[105] cloning

BBa_K1321347
BBa_K1321347 Version 1 (Component)
dCBD with N-terminal linker in RFC 25

BBa_K1159993
BBa_K1159993 Version 1 (Component)
25/25 Assembly Scar

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

BBa_I712016
BBa_I712016 Version 1 (Component)
myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site

TEV clv.
BBa_K1362452 Version 1 (Component)
TEV protease recognition/cleavage site

BBa_K1321348
BBa_K1321348 Version 1 (Component)
sfGFP fused to dCBD with linker in RFC 25

LongLinker
BBa_K243006 Version 1 (Component)
Long Linker (Gly-Gly-Ser-Gly)x3

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_K1321345
BBa_K1321345 Version 1 (Component)
NiBP fused to dCBD with linker in RFC 25

MiddleLink
BBa_K243005 Version 1 (Component)
Middle Linker ( Gly-Gly-Ser-Gly)x2

BBa_M45022
BBa_M45022 Version 1 (Component)
SNAP-25 cleavage site for Botulism neurotoxin E

BBa_K1159309
BBa_K1159309 Version 1 (Component)
19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]

BBa_K1051004
BBa_K1051004 Version 1 (Component)
Stop codon free mOrange in RFC[23] standard

BBa_K648013
BBa_K648013 Version 1 (Component)
GFP with Standard 25 Prefix/Suffix

BBa_K1159303
BBa_K1159303 Version 1 (Component)
Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]

BBa_K1319004
BBa_K1319004 Version 1 (Component)
TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

BBa_M45904
BBa_M45904 Version 1 (Component)
SNAP-25 BoNT A cleavage site

BBa_I13563
BBa_I13563 Version 1 (Component)
BioBrick site flanked by RNase sites and hairpins for use in Screening Plasmids

BBa_K1159200
BBa_K1159200 Version 1 (Component)
Splitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25]

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

BBa_K1123012
BBa_K1123012 Version 1 (Component)
His-Tag and Thrombine Cleavage Site

BBa_K407015
BBa_K407015 Version 1 (Component)
MBP + TEV cleavage site

RFC 37
BBa_K245119 Version 1 (Component)
P2

BBa_K1321351
BBa_K1321351 Version 1 (Component)
CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)

BBa_K1159108
BBa_K1159108 Version 1 (Component)
N-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]

BBa_K1159107
BBa_K1159107 Version 1 (Component)
N-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]