BBa_K622001
BBa_K622001 Version 1 (Component)
glnL: coding a nitrogen regulatory protein, NtrB

BBa_K1206000
BBa_K1206000 Version 1 (Component)
LuxOperon -> qsdA with GFP as a reporter

BBa_K1514005
BBa_K1514005 Version 1 (Component)
TOR-A TAT signaling domain for bacteria

TrzN
BBa_K1145002 Version 1 (Component)
A degrading enzyme which can degrade atrazine.

CIB1
BBa_K1592016 Version 1 (Component)
a basic helix-loop-helix protein(CIB1)

BBa_K1797012
BBa_K1797012 Version 1 (Component)
pSC101 Ori-a temperature sensitive replication origin

pucA
BBa_K227004 Version 1 (Component)
puc A coding region from R. sphaeroides

BBa_K622003
BBa_K622003 Version 1 (Component)
glnG: coding a nitrogen regulatory protein, NtrC

BBa_K1530001
BBa_K1530001 Version 1 (Component)
Homo sapiens (Human) Fibronectin Binding Protein A

BBa_K1510233
BBa_K1510233 Version 1 (Component)
A blue light regulated ccdb apoptosis gene

Pnif
BBa_K1796001 Version 1 (Component)
promoter of Paenibacillus sp. WLY78 nif gene cluster

BBa_K896905
BBa_K896905 Version 1 (Component)
a practiced part constructed by chenpowei

BBa_M31957
BBa_M31957 Version 1 (Component)
Gene V of M13K07, modified

BBa_M31960
BBa_M31960 Version 1 (Component)
Gene VII of M13K07 modified

T25 domain
BBa_K1638002 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

BBa_K1607010
BBa_K1607010 Version 1 (Component)
The coding sequence of the SCFV of anti-p185her2/neu antibody chA21

BBa_K1583055
BBa_K1583055 Version 1 (Component)
Non-coding sequence in between a gene and the next RBS

BBa_K199155
BBa_K199155 Version 1 (Component)
Measures the reverse activity of pLux in presence of pOmpC

bl
BBa_I10013 Version 1 (Component)
first half of BBa_I10003

BBa_S03491
BBa_S03491 Version 1 (Component)
Constitutive generator of 3OC6HSL

BBa_K944014
BBa_K944014 Version 1 (Component)
Cyanide Biosensor expression platform using a cyanide inducible promoter

BBa_S03734
BBa_S03734 Version 1 (Component)
hix:Carboxyl Half of Kan

BBa_B0070
BBa_B0070 Version 1 (Component)
Specialized RBS modified from that of B0036 (Brink et al.)

BBa_K1361994
BBa_K1361994 Version 1 (Component)
Curli Fiber major monomer-CsgA with his tag

BBa_K180004
BBa_K180004 Version 1 (Component)
Game of Life - Primary plasmid

BBa_M45632
BBa_M45632 Version 1 (Component)
A device to sense Vitamin D3

BBa_K1631003
BBa_K1631003 Version 1 (Component)
Tlanslational unit of Colicin Lysis Protein (for colicin-E3)

BBa_K1363001
BBa_K1363001 Version 1 (Component)
DgrB the receptor of c-di-GMP

BBa_K1113501
BBa_K1113501 Version 1 (Component)
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag

BBa_K1758370
BBa_K1758370 Version 1 (Component)
BlcR, repressor of blcABC operon, with promoter and RBS

BBa_M50037
BBa_M50037 Version 1 (Component)
Glucose Binding Protein of Thermus thermophilus

BBa_J72158
BBa_J72158 Version 1 (Component)
minimal ColE2 origin of replication

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_K1484005
BBa_K1484005 Version 1 (Component)
AmilCP chromoprotein in BBa and MoClo standard

BBa_K1179084
BBa_K1179084 Version 1 (Component)
hEF1a_Acyl-TyA-mkate-His

BBa_J70626
BBa_J70626 Version 1 (Component)
RFC12 His tagged RFP

BBa_M1719
BBa_M1719 Version 1 (Component)
Hepcidin and His-tag

BBa_J70614
BBa_J70614 Version 1 (Component)
RFC12 His tagged GFP

BBa_K864050
BBa_K864050 Version 1 (Component)
pSC101 low copy origin of replication

BBa_M12100
BBa_M12100 Version 1 (Component)
PLambda : B0034 : RFP in BioScaffold Form, a (0,5;7,2] BioScaffold part

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_K1976055
BBa_K1976055 Version 1 (Component)
T7 generator of the trypsin fragment of the Colicin E2 DNase

pSB2K4
pSB2K4 Version 1 (Component)
pSB2K3-derived BioBrick plasmid free of many restriction sites

BBa_K957004
BBa_K957004 Version 1 (Component)
nah Operon for degradation of naphthalene to salicylate

BBa_K215261
BBa_K215261 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

BBa_K1509001
BBa_K1509001 Version 1 (Component)
A bi-directional promoter affected by SmtB protein

BBa_K1675007
BBa_K1675007 Version 1 (Component)
a device to express the glutaminase1 constitutively

BBa_K1937008
BBa_K1937008 Version 1 (Component)
AF-A antifungal operon in pSBBs0K-mini

BBa_M31519
BBa_M31519 Version 1 (Component)
Modified promoter sequence of g3.