BBa_M36707
BBa_M36707 Version 1 (Component)
IclR binding sites on aceBAK promoter

BBa_M1328
BBa_M1328 Version 1 (Component)
Composite of CRT with ribosome binding site/promoter

BBa_J61103
BBa_J61103 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_J61104
BBa_J61104 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_M36100
BBa_M36100 Version 1 (Component)
Promoter, HY5 regulated (3 HY5 binding sites)

BBa_M36101
BBa_M36101 Version 1 (Component)
Promoter, HY5 regulated (2 HY5 binding sites)

BBa_M36102
BBa_M36102 Version 1 (Component)
HY5 binding site

BBa_K656001
BBa_K656001 Version 1 (Component)
sulA presequence promotor, rbs, and LexA binding site

BBa_K549038
BBa_K549038 Version 1 (Component)
Promotor prcnA of rcnA with RcnR-binding site and 50nt rcnA

BBa_K676001
BBa_K676001 Version 1 (Component)
Gyrase Binding Site from Mu Bacteriophage

BBa_K608404
BBa_K608404 Version 1 (Component)
IPTG-inducible Promoter with plastic binding domain-tagged GFP

BBa_K608405
BBa_K608405 Version 1 (Component)
plastic binding domain

BBa_K606061
BBa_K606061 Version 1 (Component)
SpoVG Ribosome Binding Site (RBS) for B. subtilis

BBa_K676011
BBa_K676011 Version 1 (Component)
Gyrase Binding Site from pSC101

BBa_K676012
BBa_K676012 Version 1 (Component)
Gyrase Binding Site from pBR322

BBa_K539711
BBa_K539711 Version 1 (Component)
ilvC (ketol-acid reductoisomerase NAD(P)-binding)

BBa_K643000
BBa_K643000 Version 1 (Component)
CDS7 cadmium sulfate binding peptide coding sequence

BBa_K615004
BBa_K615004 Version 1 (Component)
Zif268 binding site-His3-URA3 construct for one-hybrid selection

BBa_K758002
BBa_K758002 Version 1 (Component)
GAL4, this protein binds the UAS

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K737020
BBa_K737020 Version 1 (Component)
Cyanobacteria gene ribosome binding site

CBDcex
BBa_K863101 Version 1 (Component)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K737057
BBa_K737057 Version 1 (Component)
Spot42 have a weak Hfq binding site(whereas galK weaker),an endogenous terminator(unclear efficiency

BBa_K782004
BBa_K782004 Version 1 (Component)
TALA:NLS DNA binding domain

BBa_K782005
BBa_K782005 Version 1 (Component)
TALD:NLS DNA binding domain

BBa_K782006
BBa_K782006 Version 1 (Component)
TALB:NLS DNA binding domain

BBa_K782007
BBa_K782007 Version 1 (Component)
NicTAL12:NLS DNA binding domain

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157004
BBa_K157004 Version 1 (Component)
Fluoresceine-A-binding

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K165024
BBa_K165024 Version 1 (Component)
Sin3 repression domain + LexA DNA-binding domain

BBa_K165025
BBa_K165025 Version 1 (Component)
Kozak + Sin3 repression domain + LexA DNA-binding domain

BBa_K165026
BBa_K165026 Version 1 (Component)
Sin3 repression domain + Gli1 DNA-binding domain

BBa_K165027
BBa_K165027 Version 1 (Component)
Kozak + Sin3 repression domain + Gli1 DNA-binding domain

BBa_K165030
BBa_K165030 Version 1 (Component)
mCYC promoter plus Zif268-HIV binding sites

BBa_K165031
BBa_K165031 Version 1 (Component)
mCYC promoter plus LexA binding sites

BBa_K165032
BBa_K165032 Version 1 (Component)
mCYC promoter plus Gli1 binding sites

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_K112121
BBa_K112121 Version 1 (Component)
BglII endonuclease with ribosome binding site

BBa_K165033
BBa_K165033 Version 1 (Component)
YY1 binding sites + mCYC promoter

BBa_K165038
BBa_K165038 Version 1 (Component)
Gli1 binding sites + ADH1 constitutive yeast promoter

BBa_K165039
BBa_K165039 Version 1 (Component)
Zif268-HIV binding sites + ADH1 yeast promoter

BBa_K127003
BBa_K127003 Version 1 (Component)
Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator

BBa_K165040
BBa_K165040 Version 1 (Component)
Gli1 binding sites + TEF constitutive yeast promoter