BBa_K1123009BBa_K1123009 Version 1 (Component)P(KS) Protein Production Construct
BBa_K1994030BBa_K1994030 Version 1 (Component)MS2-Sigma 54 fusion protein
BBa_K543003BBa_K543003 Version 1 (Component)HNS mutant T108I protein generator
BBa_K1711000BBa_K1711000 Version 1 (Component)AFB2 auxin-binding F-box protein
ZF7BBa_K655006 Version 1 (Component)Zinc Finger Protein (ZF7) (binding site:GACGGCGCC)
BBa_K1094000BBa_K1094000 Version 1 (Component)Enhanced flavin-binding fluorescent protein (eFbFP)
BBa_K1010002BBa_K1010002 Version 1 (Component)AraC/XylS family regulatory protein(NphR)
BBa_K1123016BBa_K1123016 Version 1 (Component)DNA Coding Sequence for 1G70 Protein
JBBa_K1846008 Version 1 (Component)Bacteriophage lambda tip attachment protein J
ZF6BBa_K655005 Version 1 (Component)Zinc Finger Protein (ZF6) (binding site:GACGTAGGA)
cIBBa_K1846006 Version 1 (Component)cI repressor protein of bacteriophage lambda
BBa_K1123015BBa_K1123015 Version 1 (Component)DNA Coding Sequence for 1PJN Protein
BBa_K1076000BBa_K1076000 Version 1 (Component)fatty acid metabolism regulator protein FadR
BBa_K351008BBa_K351008 Version 1 (Component)Singlechain antibody for TB MPT51 protein
BBa_K1123014BBa_K1123014 Version 1 (Component)DNA Coding Sequence for 1ETF Protein
BBa_M45803BBa_M45803 Version 1 (Component)Tuc2009 Cro2009 gene (cro) Protein Repressor
BBa_K786032BBa_K786032 Version 1 (Component)cas3 protein in E.coli's CRISPR system
ZF8BBa_K655007 Version 1 (Component)Zinc Finger Protein (ZF8) (binding site:TGTGTGGAG)
BBa_K1012001BBa_K1012001 Version 1 (Component)Protein Phosphatase 1 with HA tag
BBa_K324001BBa_K324001 Version 1 (Component)AraC regulatory protein in RFC25 format
BBa_K1420004BBa_K1420004 Version 1 (Component)merR family transcriptional regulator, regulatory protein for mer operon
BBa_I12243BBa_I12243 Version 1 (Component)Test of AraC protein generator
BBa_I12241BBa_I12241 Version 1 (Component)Test of TetR protein generator
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K316030BBa_K316030 Version 1 (Component)LytC cell wall anchoring protein for B. subtilis
pSB1AC3FBBa_J18901 Version 1 (Component)pSB1AC3F construction plasmid for protein fusion BioBricks
pSB1AT3FBBa_J18903 Version 1 (Component)pSB1AT3F construction plasmid for protein fusion BioBricks
pSB1AK3FBBa_J18902 Version 1 (Component)pSB1AK3F construction plasmid for protein fusion BioBricks
BBa_K1159004BBa_K1159004 Version 1 (Component)Human protein phosphatase 1 (PP1) in RFC[25]
BBa_I12043BBa_I12043 Version 1 (Component)Activator for BL oscillator with reporter protein
BBa_I20241BBa_I20241 Version 1 (Component)Double protein generator of lacI and T7 RNAP
BBa_K322149BBa_K322149 Version 1 (Component)Bacterial luciferase (luxAB) blue shifted by lumazine protein
BBa_J32009BBa_J32009 Version 1 (Component)4HT Dependent Intein
BBa_K311003BBa_K311003 Version 1 (Component)Bacterial Microcompartment Proteins
BBa_K1631021BBa_K1631021 Version 1 (Component)Plac -rbs- Colicin Immunity protein -d.term
BBa_M36626BBa_M36626 Version 1 (Component)AFP Pre-Protein with GFP Tag
BBa_K1631015BBa_K1631015 Version 1 (Component)Colicin Lysis Protein Generator (medium, constitutive)
BBa_K1417000BBa_K1417000 Version 1 (Component)Red Fluorescent Protein - Spacer - Spinach Aptamer
BBa_M1543BBa_M1543 Version 1 (Component)Part 1 DNA replication and repair protein RecF
BBa_K174008BBa_K174008 Version 1 (Component)SmtA metallothionein protein with CotC and Gfp fusion
BBa_K1976029BBa_K1976029 Version 1 (Component)Colicin E2 immunity protein with RBS
BBa_K1350002BBa_K1350002 Version 1 (Component)Promotor+RBS(BBa_K608002) + kil protein(BBa_K1350001)
BBa_K1975007BBa_K1975007 Version 1 (Component)PhaB fused to Red Fluorescent Protein
BBa_K584027BBa_K584027 Version 1 (Component)Constitutive_Promoter + INP(Ice Nucleating Protein Generator)
BBa_K516012BBa_K516012 Version 1 (Component)luxI protein generator TERM- RBS+(B0032)
Si-tag1+liBBa_K1592013 Version 1 (Component)LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273aa) + YLcwp3
ArtEBBa_K1659088 Version 1 (Component)Artilysin Art-E, an antibacterial fusion protein
BBa_J70620BBa_J70620 Version 1 (Component)RFC12 Maltose Binding Protein Internal Domain [malE]