BBa_K817016
BBa_K817016 Version 1 (Component)
parS site

BBa_K817017
BBa_K817017 Version 1 (Component)
pSB2K3 with parS site

BBa_K737020
BBa_K737020 Version 1 (Component)
Cyanobacteria gene ribosome binding site

BBa_K737037
BBa_K737037 Version 1 (Component)
J23106,P0412,R0011,ClaI site,SalI site,truncated Spot42

BBa_K737057
BBa_K737057 Version 1 (Component)
Spot42 have a weak Hfq binding site(whereas galK weaker),an endogenous terminator(unclear efficiency

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K165030
BBa_K165030 Version 1 (Component)
mCYC promoter plus Zif268-HIV binding sites

BBa_K165031
BBa_K165031 Version 1 (Component)
mCYC promoter plus LexA binding sites

BBa_K165032
BBa_K165032 Version 1 (Component)
mCYC promoter plus Gli1 binding sites

BBa_K112121
BBa_K112121 Version 1 (Component)
BglII endonuclease with ribosome binding site

BBa_K165033
BBa_K165033 Version 1 (Component)
YY1 binding sites + mCYC promoter

BBa_K165038
BBa_K165038 Version 1 (Component)
Gli1 binding sites + ADH1 constitutive yeast promoter

BBa_K165039
BBa_K165039 Version 1 (Component)
Zif268-HIV binding sites + ADH1 yeast promoter

BBa_K165040
BBa_K165040 Version 1 (Component)
Gli1 binding sites + TEF constitutive yeast promoter

BBa_K165041
BBa_K165041 Version 1 (Component)
Zif268-HIV binding sites + TEF constitutive yeast promoter

BBa_K165042
BBa_K165042 Version 1 (Component)
Gli1 binding sites + MET25 inducible yeast promoter

BBa_K165043
BBa_K165043 Version 1 (Component)
Zif268-HIV binding sites + MET25 constitutive yeast promoter

BBa_K165045
BBa_K165045 Version 1 (Component)
pGAL1+ LexA bindingsites

BBa_K089005
BBa_K089005 Version 1 (Component)
-35 to Tc start site of phaC

BBa_K089006
BBa_K089006 Version 1 (Component)
-663 to Tc start site of phaC

BBa_K122021
BBa_K122021 Version 1 (Component)
Cassette3: Constitutive TAL with integration site

BBa_K116012
BBa_K116012 Version 1 (Component)
Ribosomal binding site + yaaU

BBa_K116013
BBa_K116013 Version 1 (Component)
Lac promoter + ribosomal binding site + yaaU

BBa_K112142
BBa_K112142 Version 1 (Component)
attR2 recombination site-reverse orientation

BBa_K112141
BBa_K112141 Version 1 (Component)
attR2 recombination site

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_J70305
BBa_J70305 Version 1 (Component)
GFP coding device switched on by IPTG (PsrI site removed)

BBa_I715071
BBa_I715071 Version 1 (Component)
Lac promoter with Ribosomal Binding Site

BBa_J70306
BBa_J70306 Version 1 (Component)
Similar to S03621, except PsrI sites (some portions of B0010, B0012) removed

BBa_J70368
BBa_J70368 Version 1 (Component)
I13502 or K093005 (B0034+E1010) rfp without PsrI sites

BBa_K223002
BBa_K223002 Version 1 (Component)
RBS + SoxR- ribosome binding site and SoxR gene

BBa_K175002
BBa_K175002 Version 1 (Component)
I-PpoI homing endonuclease site

AraC O2
BBa_R0081 Version 1 (Component)
Inhibitor (AraC loop attachment with O2 site)

I-SceI RS
BBa_K175027 Version 1 (Component)
I-SceI restriction site

BBa_K182103
BBa_K182103 Version 1 (Component)
Composite part of K182100 and K182102

BBa_K200009
BBa_K200009 Version 1 (Component)
DpnII Composite

BBa_K200010
BBa_K200010 Version 1 (Component)
TaqI Composite

VR3 bindin
BBa_K176037 Version 1 (Component)
Reverse primer VR3 (K176047) binding site

VR4 bindin
BBa_K176038 Version 1 (Component)
Reverse primer VR4 (K176048) binding site

VR5 bindin
BBa_K176039 Version 1 (Component)
Reverse primer VR5 (K176049) binding site

VR6 bindin
BBa_K176040 Version 1 (Component)
Reverse primer VR6 (K176050) binding site

VR7 bindin
BBa_K176041 Version 1 (Component)
Reverse primer VR7 (K176051) binding site

VR8 bindin
BBa_K176042 Version 1 (Component)
Reverse primer VR8 (K176052) binding site

VR9 bindin
BBa_K176043 Version 1 (Component)
Reverse primer VR9 (K176053) binding site

VR10 bindi
BBa_K176044 Version 1 (Component)
Reverse primer VR10 (K176054) binding site

VR11 bindi
BBa_K176045 Version 1 (Component)
Reverse primer VR11 (K176055) binding site

VR12 bindi
BBa_K176046 Version 1 (Component)
Reverse primer VR12 (K176056) binding site