BBa_K1935014
BBa_K1935014 Version 1 (Component)
White Collar 1 under the control of pU6 promoter in C.elegans (pU6::WC1)

BBa_K2082232
BBa_K2082232 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)

xis
BBa_K112205 Version 1 (Component)
{rbs.xis!} The xis gene with native rbs site and stop codon; assembly standard 21

BBa_M11206
BBa_M11206 Version 1 (Component)
mNarK promoter + RFP to monitor oxygen monitors in S. cerevisiae

BBa_J24016
BBa_J24016 Version 1 (Component)
Toggle Switch to Immobilize E. Coli

BBa_M45632
BBa_M45632 Version 1 (Component)
A device to sense Vitamin D3

16s rRNA
BBa_C0101 Version 1 (Component)
16s rRNA (non-standard 5' end, matched to B0035)

BBa_K540000
BBa_K540000 Version 1 (Component)
<i>rcn-csgBAEFG</i>, over-induces adherence in response to cobalt

BBa_K730000
BBa_K730000 Version 1 (Component)
The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.

BBa_K1113502
BBa_K1113502 Version 1 (Component)
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag

BBa_K1165012
BBa_K1165012 Version 1 (Component)
Ethylene Synthesis Cassette (SAM Synthetase, ACC Oxidase, ACC Synthase) under the Control of pTet Pr

BBa_J24015
BBa_J24015 Version 1 (Component)
Toggle Switch to Immobilize E. Coli

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J24017
BBa_J24017 Version 1 (Component)
Toggle Switch to Immobilize E. Coli

BBa_J26010
BBa_J26010 Version 1 (Component)
LuxR + AHL used to turn on GFP

BBa_J49008
BBa_J49008 Version 1 (Component)
Rooney to Tea-time Module Ver. 1.0

BBa_K1430008
BBa_K1430008 Version 1 (Component)
Tool to tag yeast proteins with mRFP

BBa_K553004
BBa_K553004 Version 1 (Component)
OC12 HLA in response to OC8 HLA

BBa_K1321355
BBa_K1321355 Version 1 (Component)
sfGFP fused to CBDcex driven by T7

PhaA-CFP
BBa_K1975004 Version 1 (Component)
Beta-Ketothiolase fused to Cyan Fluorescent Protein

BBa_K1321354
BBa_K1321354 Version 1 (Component)
sfGFP fused to CBDclos driven by T7

BBa_K1430007
BBa_K1430007 Version 1 (Component)
Tool to tag yeast proteins with GFP

BBa_K1321359
BBa_K1321359 Version 1 (Component)
sfGFP fused to dCBD driven by LacI

BBa_J24666
BBa_J24666 Version 1 (Component)
Toggle Switch to Immobilize E. Coli

ADH
BBa_M36200 Version 1 (Component)
ADH; converts ethanol to/from acetaldehyde; no STOP codon

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_I4285
BBa_I4285 Version 1 (Component)
Trans-splicing Tetrahymena ribozyme to remove LVA tag

BBa_K1212003
BBa_K1212003 Version 1 (Component)
TALe Binding Site 1 (specific to TAL repressor 1)

BBa_K1778003
BBa_K1778003 Version 1 (Component)
RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues.

BBa_K315005
BBa_K315005 Version 1 (Component)
variant reverse lox site with 3 base changes in the spacer region (loxm2 reverse)

loxBri (R)
BBa_K315006 Version 1 (Component)
LoxBri (R)variant reverse lox site with 3 base changes in the spacer region

BBa_K737070
BBa_K737070 Version 1 (Component)
phoR, a histine kinase coding gene involved in phoBR regulon, is the regulatory protein that could a

BBa_M31337
BBa_M31337 Version 1 (Component)
section of M13.1 from HpaI to BamHI (old)

BBa_K371051
BBa_K371051 Version 1 (Component)
pSB1C5 (1bp mutation of pSB1C3 to remove SacI)

BBa_K2060000
BBa_K2060000 Version 1 (Component)
CRISPR-Cas9 guide RNA targeting to 16S RNA

EHEC_TALE1
BBa_K1189032 Version 1 (Component)
EHEC TALE1 (binds to 19bp of stx2 gene)

BBa_M31520
BBa_M31520 Version 1 (Component)
g8 modified to be independent of g3 promoter

rubB
BBa_K826006 Version 1 (Component)
rubredoxin reductase act with rubA to transport electron

BBa_M31511
BBa_M31511 Version 1 (Component)
HpaI to BamHI section of M13K07 genome - refactor

BBa_K422001
BBa_K422001 Version 1 (Component)
Archeal light receptor fused to bacterial chemotaxis transducer

BBa_K1928003
BBa_K1928003 Version 1 (Component)
toehold switch sensor to detect HCV 1b RNA

BBa_K360114
BBa_K360114 Version 1 (Component)
Primer to change RBS of P. pyralis luciferase

BBa_K1928001
BBa_K1928001 Version 1 (Component)
toehold switch sensor to detect HCV 1b RNA

BBa_K1906020
BBa_K1906020 Version 1 (Component)
ribothermometer modified A1 to work as positive cpntrol

BBa_K2116000
BBa_K2116000 Version 1 (Component)
RBS designed to achieve maximal expression of EsaR